Large-scale gene trapping in C57BL/6N mouse embryonic stem cells
Large-scale gene trapping in C57BL/6N mouse embryonic stem cells
复制标题
DOI：
10.1101/gr.078352.108
复制
发表时间：
2008-10-01
期刊：
影响因子：
7
通讯作者：
Abuin, Alejandro
中科院分区：
文献类型：
--
作者：
Hansen, Gwenn M.;Markesich, Diane C.;Abuin, Alejandro
我们报道了在C57BL/6N遗传背景下构建和分析的一个小鼠基因捕获突变体资源，其中包含超过350,000个带有序列标签的胚胎干细胞（ES）克隆。我们还证明了这些ES细胞克隆能够参与生殖系并产生基因敲除小鼠。每个突变克隆都由一个代表精确插入位置的基因组序列标签来识别，从而能够准确预测致突变性，并对突变等位基因进行直接基因分型。在超过10,000个基因中已经鉴定出突变，并且显示出对第一内含子的偏向性。这些被捕获的ES细胞系可从德克萨斯A&M基因组医学研究所获取，可供科学界使用。
We report the construction and analysis of a mouse gene trap mutant resource created in the C57BL/6N genetic background containing more than 350,000 sequence-tagged embryonic stem (ES) cell clones. We also demonstrate the ability of these ES cell clones to contribute to the germline and produce knockout mice. Each mutant clone is identified by a genomic sequence tag representing the exact insertion location, allowing accurate prediction of mutagenicity and enabling direct genotyping of mutant alleles. Mutations have been identified in more than 10,000 genes and show a bias toward the first intron. The trapped ES cell lines, which can be requested from the Texas A&M Institute for Genomic Medicine, are readily available to the scientific community.