Identification and analysis of U5 snRNA variants in Drosophila
Identification and analysis of U5 snRNA variants in Drosophila
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DOI：
10.1261/rna.2141505
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发表时间：
2005-10-01
期刊：
影响因子：
4.5
通讯作者：
Doudna, JA
中科院分区：
文献类型：
--
作者：
Chen, L;Lullo, DJ;Doudna, JA
剪接体RNA的不同亚型可能参与调节真核细胞中的前信使RNA剪接。在一个大规模的努力，以确定小的非编码RNA在果蝇中，我们分离出一个美国snRNA样分子含有一个5'节段相同的经典（主要）美国snRNA，但有一个变异的Sm结合位点和一个独特的3'发夹序列。基于这一发现，通过与US的5'半中的不变环的序列相似性，在果蝇基因组中鉴定出另外6个相似的US snRNA样序列。有趣的是，虽然所有这些变体都在体内表达，但在果蝇发育过程中，每个变体都显示出不同的时间表达谱，其中一个主要在果蝇头中表达。RNP颗粒内这些US snRNA变体的存在表明它们在剪接中的作用，并暗示可能与发育和组织特异性基因表达的调节有关。
Distinct isoforms of spliceosomal RNAs may be involved in regulating pre-messenger RNA splicing in eukaryotic cells. During a large-scale effort to identify small noncoding RNAs in Drosophila, we isolated a US snRNA-like molecule containing a 5' segment identical to that of the canonical (major) US snRNA but with a variant Sm binding site and a distinct 3' hairpin sequence. Based on this finding, another six similar US snRNA-like sequences were identified within the Drosophila genome by sequence similarity to the invariant loop in the 5' half of US. Interestingly, although all of these variants are expressed in vivo, each shows a distinct temporal expression profile during Drosophila development, and one is expressed primarily in fly heads. The presence of these US snRNA variants within RNP particles suggests their role in splicing and implies a possible connection to regulation of developmental and tissue-specific gene expression.