Microarray analysis of differentially expressed transcripts in porcine intestinal epithelial cells (IPEC-J2) infected with porcine sapelovirus as a model to study innate immune responses to enteric viruses
Microarray analysis of differentially expressed transcripts in porcine intestinal epithelial cells (IPEC-J2) infected with porcine sapelovirus as a model to study innate immune responses to enteric viruses
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DOI：
10.1007/s00705-013-1638-2
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发表时间：
2013-07-01
影响因子：
2.7
通讯作者：
Li, Jian
中科院分区：
文献类型：
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作者：
Lan, Daoliang;Tang, Cheng;Li, Jian
局部肠粘膜是动物体内最大的粘膜免疫系统，在抵抗肠道病原菌感染中起着重要作用。然而，肠粘膜的分子抗病毒机制仍然知之甚少。在这项研究中，我们筛选和鉴定差异表达的转录本（PSV）的猪肠上皮细胞（IPEC-J2）感染猪萨佩罗病毒，使用微阵列分析。共筛选出2298个差异表达基因。这些基因参与了许多物理系统和分子通路，特别是一些先天免疫相关通路。结果表明，PSV感染IPEC-J2细胞后，诱导产生大量的I型干扰素，并激活了相关的干扰素效应通路。先天免疫受体的三条途径，包括Toll样受体、NOD样受体和RIG-I样受体，也被激活。然后通过MHCI和MHCII途径加工和呈递抗原。有趣的是，我们发现伊加的分泌网络在PSV感染的早期被激活。两个外源性和内源性凋亡途径也被激活在PSV感染。结果揭示了基因转录的变化，特别是与IPEC-J2细胞中PSV感染相关的先天免疫途径基因的变化。
The local intestinal mucosa, the largest mucosal immune system in animals, plays an important role in resistance against intestinal pathogen infection. However, the molecular antiviral mechanisms of the intestinal mucosa remain poorly understood. In this study, we screened and identified differentially expressed transcripts in (PSV) porcine intestinal epithelial cells (IPEC-J2) infected with porcine sapelovirus using microarray analysis. A total of 2298 differentially expressed genes were screened at four time points during PSV infection. These genes were involved in numerous physical systems and molecular pathways, and particularly, some innate immune-associated pathways were significant. The results showed that large amounts of type I interferon were induced, and the related interferon effect pathway was activated when IPEC-J2 cells were infected with PSV. Three pathways of innate immune receptors, including Toll-like, NOD-like, and RIG-I-like receptors, were also activated. The antigen was then processed and presented through the MHCI and MHCII pathways. Interestingly, we found that the secretion network of IgA was activated in the early stage of PSV infection. Two exogenous and endogenous apoptosis pathways were also activated during PSV infection. The results revealed changes in gene transcription, particularly those of innate immune pathway genes that were associated with PSV infection in IPEC-J2 cells.