E2F-induced S phase requires cyclin E
E2F-induced S phase requires cyclin E
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DOI：
10.1101/gad.10.19.2505
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发表时间：
1996-10-01
影响因子：
10.5
通讯作者：
OFarrell, PH
中科院分区：
文献类型：
--
作者：
Duronio, RJ;Brook, A;OFarrell, PH
异源二聚体转录因子E2 F和G（1）细胞周期蛋白cyclin E都是后生动物细胞周期开始时G（1）-S转换所必需的。已经确定细胞周期蛋白E可以作为E2 F的上游激活剂。除了这一行动，我们在这里表明，细胞周期蛋白E在DNA复制激活E2 F不同的重要作用。我们已经创建了转基因果蝇能够诱导，异位生产E2 F活性。果蝇E2 F亚基dE 2F和dDP在胚胎中的同时过表达刺激了包括cyclin E在内的多个E2 F靶基因的表达，也引起了S期的启动。cyclin E的突变阻止了dE 2 E/dDP过表达后S期的启动，而不影响靶基因的表达诱导。因此，E2 F指导的转录不能绕过果蝇胚胎中细胞周期蛋白E的丢失。
Both the heterodimeric transcription factor, E2F, and the G(1) cyclin, cyclin E, are required for the G(1)-S transition at the start of the metazoan cell cycle. It has been established that cyclin E can act as an upstream activator of E2F. In addition to this action, we show here that cyclin E has an essential role in DNA replication distinct from activating E2F. We have created transgenic Drosophila capable of inducible, ectopic production of E2F activity. Simultaneous overexpression of both Drosophila E2F subunits, dE2F and dDP, in embryos stimulated the expression of multiple E2F-target genes including cycIin E, and also caused the initiation of S phase. Mutation of cycIin E prevented the initiation of S phase after overexpression of dE2E/dDP without affecting induction of target gene expression. Thus, E2F-directed transcription cannot bypass loss of cyclin E in Drosophila embryos.