Emergence of a novel multidrug-resistant Pseudomonas aeruginosa strain producing IMP-type metallo-β-lactamases and AAC(6′)-Iae in Japan
Emergence of a novel multidrug-resistant Pseudomonas aeruginosa strain producing IMP-type metallo-β-lactamases and AAC(6′)-Iae in Japan
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DOI：
10.1016/j.ijantimicag.2012.01.020
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发表时间：
2012-06-01
影响因子：
10.8
通讯作者：
Kirikae, Teruo
中科院分区：
文献类型：
--
作者：
Kitao, Tomoe;Tada, Tatsuya;Kirikae, Teruo
多药耐药（MDR）铜绿假单胞菌分离株产生imp型金属β -内酰胺酶（MBLs）和氨基糖苷6′- n -乙酰转移酶[AAC（6′）-Iae]的出现已成为日本医疗环境中的一个严重问题。2009年8月至2010年4月，共从日本144家医院的患者中分离出217株耐多药铜绿假单胞菌，其中145株（66.8%）经免疫层析检测为imp型MBLs和AAC（6′）-Iae阳性。聚合酶链反应（PCR）结果显示，这些分离株blaIMP和aac（6’）-Iae基因均呈阳性。当用脉冲场凝胶电泳（PFGE）分析这些imp型MBL-和AAC（6’）- iae -产菌株时，检测到两个簇（I和II）。大部分分离株（88.3%,128/145）归为I类，除1株分离株为ST991和O3型外，其余多位点序列为ST235型和O11型。分离株主要来自泌尿道（82/145,56.6%）和呼吸道（58/145,40.0%）。属于聚类I的分离株的流行病学特性与先前在日本报道的耐多药铜绿假单胞菌分离株相似。其余16株属于ⅱ类，PFGE模式相同，多位点序列为ST991型和O18型；所有分离株均来自呼吸道。属于集群II的分离株的特性以前没有被描述过，这表明一种新的imp型MBL-和AAC(6')- iae产生铜绿假单胞菌菌株正在日本出现。属于这两个群集的分离物是从该国不同地区分离出来的。(c) 2012年Elsevier B.V.与International Society of Chemotherapy。版权所有。
The emergence of multidrug-resistant (MDR) Pseudomonas aeruginosa isolates producing IMP-type metallo-beta-lactamases (MBLs) and aminoglycoside 6'-N-acetyltransferase [AAC(6')-Iae] has become a serious problem in medical settings in Japan. A total of 217 MDR P. aeruginosa isolates were obtained from August 2009 to April 2010 from patients at 144 hospitals in Japan, of which 145 (66.8%) were positive for IMP-type MBLs and AAC(6')-Iae when tested with an immunochromatographic assay. Polymerase chain reaction (PCR) showed that these isolates were also positive for blaIMP and aac(6')-Iae genes. When these IMP-type MBL- and AAC(6')-Iae-producing isolates were analysed by pulsed-field gel electrophoresis (PFGE), two clusters (I and II) were detected. Most of the isolates (88.3%; 128/145) were grouped under cluster I and had multilocus sequence type ST235 and serotype O11, except for one isolate that was ST991 and serotype O3. The isolates were mainly isolated from the urinary tract (82/145; 56.6%) and respiratory tract (58/145; 40.0%). The epidemiological properties of the isolates belonging to cluster I were similar to those of MDR P. aeruginosa isolates that have been previously reported in Japan. The remaining 16 isolates belonged to cluster II, had identical PFGE patterns and were multilocus sequence type ST991 and serotype O18; all of these isolates were isolated from the respiratory tract. The properties of isolates belonging to cluster II have not been previously described, indicating that a novel IMP-type MBL- and AAC(6')-Iae producing P. aeruginosa strain is emerging in Japan. Isolates belonging to both clusters were isolated from different parts of the country. (c) 2012 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.