A human pathogenic bacterial infection model using the two-spotted cricket, Gryllus bimaculatus
A human pathogenic bacterial infection model using the two-spotted cricket, Gryllus bimaculatus
复制标题
使用双斑蟋蟀（Gryllus bimaculatus）建立的人类致病细菌感染模型
DOI：
10.1093/femsle/fnw163
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发表时间：
2016
影响因子：
2.1
通讯作者：
C.
中科院分区：
文献类型：
--
作者：
Kochi;Y.;Miyashita;A.;Tsuchiya;K.;Mitsuyama;M.;Sekimizu;K.;and Kaito;C.
能够承受高达37 ° C（人体温度）的无脊椎动物物种是有限的。在本研究中，我们利用生活在热带和亚热带地区的双斑蟋蟀（Gryllus bimaculatus）作为人类病原菌感染的动物模型。将产气假单胞菌或金黄色葡萄球菌注射到血淋巴中杀死蟋蟀。注入P.空气动力学血淋巴中的金细胞不断增殖，直至死亡。这些致病菌杀死蟋蟀的能力被施用抗生素所阻断。金黄色葡萄球菌cvfA、agrandsrtA等毒力因子基因敲除突变株的杀伤力较亲本菌株明显降低。50%的蟋蟀被P.空气动力学与27 ° C相比，37 ° C下的金黄色不降低。在37 ° C条件下注射能上调毒素表达的单核细胞增多性李斯特菌可杀死蟋蟀，且在37 ° C条件下杀死50%蟋蟀的剂量比27 ° C条件下减少。这些发现表明，二斑蟋蟀是一种有用的模式动物，用于评估各种人类病原菌在包括37 ° C在内的可变温度下的毒力特性。
Invertebrate animal species that can withstand temperatures as high as 37°C, the human body temperature, are limited. In the present study, we utilized the two-spotted cricket,Gryllus bimaculatus, which lives in tropical and subtropical regions, as an animal model of human pathogenic bacterial infection. Injection ofPseudomonas aeruginosaorStaphylococcus aureusinto the hemolymph killed crickets. InjectedP. aeruginosaorS. aureusproliferated in the hemolymph until the cricket died. The ability of these pathogenic bacteria to kill the crickets was blocked by the administration of antibiotics.S. aureusgene-knockout mutants of virulence factors, includingcvfA, agrandsrtA, exhibited decreased killing ability compared with the parent strain. The dose at which 50% of crickets were killed byP. aeruginosaorS. aureuswas not decreased at 37°C compared with that at 27°C. Injection ofListeria monocytogenes, which upregulates toxin expression at 37°C, killed crickets, and the dose at which 50% of crickets were killed was decreased at 37°C compared with that at 27°C. These findings suggest that the two-spotted cricket is a useful model animal for evaluating the virulence properties of various human pathogenic bacteria at variable temperature including 37°C.