Ingestion and killing of Listeria monocytogenes by blood and milk phagocytes from mastitic and normal cattle.
Ingestion and killing of Listeria monocytogenes by blood and milk phagocytes from mastitic and normal cattle.
复制标题
乳腺牛和正常牛的血液和乳汁吞噬细胞摄入并杀死单核细胞增多性李斯特菌。
DOI：
10.1128/jcm.27.5.812-817.1989
复制
发表时间：
1989
影响因子：
9.4
通讯作者：
Schultz,RD
中科院分区：
文献类型：
--
作者：
Czuprynski,CJ;Noel,EJ;Doyle,MP;Schultz,RD
食用受沙门氏菌污染的乳制品导致的人类沙门氏菌病正在成为一个重大的公共卫生问题。有必要更好地了解单核细胞增生李斯特菌诱导的奶牛乳房炎的发病机制所涉及的过程。在本报告中，我们描述了在体外相互作用的L。单核细胞增多症与牛血和牛奶白细胞。实验L.单核细胞增生性乳腺炎导致受感染奶牛的乳汁中嗜中性粒细胞的快速和显著增加。感染和未感染的奶牛的血液中性粒细胞、单核细胞和乳汁白细胞容易摄入L。单核细胞增多症的血清调理素的存在下。这些白细胞也杀死了一部分摄入的白细胞。摄入马唐诱发血液中性粒细胞的强烈化学发光反应和血液单核细胞的反应相对较弱。乳汁白细胞的摄入、杀伤和化学发光与中性粒细胞的百分比直接相关。健康供体牛的血液中性粒细胞摄入并杀死L。当白细胞耗竭的牛奶和乳清从乳腺炎奶牛调理素的唯一来源，单核细胞增多症，虽然较少的Escheriae摄入比正常牛血清时，存在。这些结果表明，牛血和牛奶的吞噬细胞，像其他哺乳动物的血液和炎症吞噬细胞，可以摄取和杀死L。体外单核细胞增生。
Human listeriosis resulting from consumption of listeria-contaminated dairy products is emerging as a significant public health concern. There is a need to understand better the processes involved in the pathogenesis of Listeria monocytogenes-induced bovine mastitis. In the present report, we describe the results of the in vitro interaction of L. monocytogenes with bovine blood and milk leukocytes. Induction of an experimental L. monocytogenes mastitis resulted in a rapid and dramatic increase in neutrophils in the milk of infected cows. Blood neutrophils and mononuclear cells and milk leukocytes from listeria-infected and uninfected cows readily ingested L. monocytogenes in the presence of serum opsonins. These leukocytes also killed a portion of the ingested listeriae. Ingestion of listeriae evoked a vigorous chemiluminescence response by blood neutrophils and a relatively weak response by blood mononuclear cells. Ingestion, killing, and chemiluminescence by milk leukocytes were directly related to the percentage of neutrophils that were present. Blood neutrophils from healthy donor cattle ingested and killed L. monocytogenes when leukocyte-depleted milk and whey from mastitic cows were the sole sources of opsonins, although fewer listeriae were ingested than when normal bovine serum was present. These results indicate that bovine blood and milk phagocytes, like blood and inflammatory phagocytes from other mammalian species, can ingest and kill L. monocytogenes in vitro.