Metal decorated graphene nanosheets as immobilization matrix for amperometric glucose biosensor
Metal decorated graphene nanosheets as immobilization matrix for amperometric glucose biosensor
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DOI：
10.1016/j.snb.2009.11.022
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发表时间：
2010-03-04
影响因子：
8.4
通讯作者：
Ramaprabhu, S.
中科院分区：
文献类型：
--
作者：
Baby, Tessy Theres;Aravind, S. S. Jyothirmayee;Ramaprabhu, S.
利用铂金（Pt-Au）和金（Au）纳米颗粒间隔层装饰石墨烯纳米片，制备了电流型葡萄糖生物传感器。通过氧化石墨的剥离制备了功能化石墨烯（f-G）薄片，并使用简单的化学还原方法在其表面装饰了晶体(Pt-Au)/Au金属纳米颗粒。采用物理吸附的方法将葡萄糖氧化酶（GOD）固定在nafon溶解的金属纳米颗粒分散的石墨烯f-G-(Pt-Au)和f-G-(Au)电极上。所得的生物电极保留其生物催化活性，并提供快速和敏感的葡萄糖定量。在pH 7.0的磷酸盐缓冲液中，在+0.8 V的最佳电位下，用电化学方法研究了该生物传感器的性能。制备的f-G-(Au)基葡萄糖生物传感器表现出最佳的传感性能，线性响应高达30 mM，检测限为1 μ m。使用（Pt-Au）和（Au）纳米颗粒间隔物消除f-G的再堆积，从而增加了表面面积和葡萄糖传感性能。(C) 2009 Elsevier B.V.版权所有
Amperometric glucose biosensors have been fabricated by using platinum-gold (Pt-Au) and gold (Au) nanoparticle spacers decorated graphene nanosheets. Functionalized graphene (f-G) sheets have been prepared by exfoliation of graphitic oxide and it has been decorated with crystalline (Pt-Au)/Au metal nanoparticles using a simple chemical reduction method. The immobilization of glucose oxidase (GOD) over Nafion-solubilized metal nanoparticles dispersed graphene f-G-(Pt-Au) and f-G-(Au) electrode has been achieved by physical adsorption. The resultant bioelectrode retains its biocatalytic activity and offers fast and sensitive glucose quantification. The performances of the biosensor have been investigated by electrochemical method at an optimum potential of +0.8 V in pH 7.0 phosphate buffer. The fabricated f-G-(Au) based glucose biosensor exhibits best sensing performance with a linear response up to 30 mM with an excellent detection limit of 1 mu M. The elimination of restacking of f-G by using (Pt-Au) and (Au) nanoparticle spacers resulted in the increase in the surface area and glucose sensing performance. (C) 2009 Elsevier B.V. All rights reserved.