U1, U2 and U5 small nuclear RNAs are found in plants cells. Complete nucleotide sequence of the U5 RNA family from pea nuclei.
U1, U2 and U5 small nuclear RNAs are found in plants cells. Complete nucleotide sequence of the U5 RNA family from pea nuclei.
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U1、U2 和 U5 小核 RNA 存在于植物细胞中。
DOI：
10.1093/nar/11.24.8583
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发表时间：
1983
影响因子：
14.9
通讯作者：
R. Lührmann
中科院分区：
文献类型：
--
作者：
A. Krol;J. Ebel;J. Rinke;R. Lührmann
U1、U2和U 5 RNA用特异于2，2，7-三甲基鸟苷的抗体从豌豆核中分离。给出了豌豆U1和U2 snRNA的3 '端半部分的核苷酸序列以及分离的六种U 5 RNA变体中的五种的完整序列。大量的U 5变异体表明它们是由一个包含至少六个不同基因的多基因家族编码的。所有豌豆U 5 RNA的二级结构相似，尽管植物和脊椎动物U 5 RNA之间的序列保守程度低至35%，但两种RNA组的二级结构几乎相同。所有来自豌豆的snRNA种类U1、U2和U 5共享一个结构域，即所谓的结构域A，这也是所有动物snRNA U1、U2、U4和U 5所共有的。此外，豌豆和脊椎动物U 5 RNA中保守的22个连续核苷酸的块，其中11个核苷酸构成具有大量转录后修饰的发夹环。我们建议，这个发夹环的保护，与域A，是首要的重要性，在植物和动物细胞中的U 5 RNA的功能。
U1, U2 and U5 RNAs were isolated from pea nuclei with antibody specific for 2,2,7-trimethylguanosine. The nucleotide sequence of the 3'-terminal halves of pea U1 and U2 snRNAs and the complete sequence of five out of the six U5 RNA variants isolated is given. The high number of U5 variants suggest they are encoded by a multigene family containing at least six different genes. Similar secondary structures could be derived for all of the pea U5 RNAs and although the degree of sequence conservation between plant and vertebrate U5 RNAs is as low as 35%, nearly identical secondary structures can be proposed for both RNA groups. All the snRNA species U1, U2 and U5 from pea share a structural domain, the so-called domain A, which is also common to all animal snRNA U1, U2, U4 and U5. Furthermore, a block of 22 consecutive nucleotides is conserved among pea and vertebrate U5 RNAs, from which 11 nucleotides constitute a hairpin-loop with a high number of posttranscriptional modifications. We propose that conservation of this hairpin-loop, together with domain A, is of prime importance for the functioning of U5 RNAs in plant and animal cells.