Migratory pathway of sympathetic preganglionic neurons in normal and reeler mutant mice.
Migratory pathway of sympathetic preganglionic neurons in normal and reeler mutant mice.
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正常和 reeler 突变小鼠交感神经节前神经元的迁移途径。
DOI：
10.1002/cne.10634
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Yip,JosephW
中科院分区：
文献类型：
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作者：
Yip,YeePing;Capriotti,Christine;Yip,JosephW
我们之前的研究表明，脊髓交感神经节前神经元（SPN）的迁移在该突变体中受到影响。本研究使用形态计量学分析来描述和比较SPN在进展发育阶段的位置，提供了SPN在存在或缺乏该基因时如何迁移的详细信息。我们发现SPN从神经上皮向腹外侧脊髓的初始迁移（在E11.5之前）在对照（野生型和杂合子）和小鼠中是相似的。然而，随着发育的进展（E12.5-E15.5），对照小鼠的SPN向脊髓的中外侧区域背侧迁移，其中80%的SPN在此定居形成中外侧柱（IML）；其余的则向内侧迁移至内腭和中央椎管之间的位置。实际上，80%的SPN向背内侧迁移，聚集在中央椎管周围，其余分布在中央椎管和脊髓中间外侧区域之间。本研究还探讨了对照组小鼠SPN、Reelin和径向胶质纤维之间的关系。共聚焦显微镜研究表明，在初始迁移过程中，对照组和裸鼠的SPN都与腹外侧脊髓的径向胶质纤维密切相关。对照小鼠的大部分SPN然后沿垂直于径向胶质纤维的方向背侧迁移，形成IML。相比之下，大多数SPN以与径向胶质纤维相同的方向向中央管迁移，而不是背侧迁移形成IML。对这些结果的一种可能解释是，Reelin的作用是阻止SPN沿径向胶质纤维向中央管的反向迁移。[j] .中华神经医学杂志，2006,31(4):559 - 559。©2003 Wiley‐Liss, Inc。
Our previous study showed that the migration of sympathetic preganglionic neurons (SPN) in the spinal cord is affected in thereelermutant. The present study, using morphometric analysis to describe and compare the location of SPN at progressive developmental stages, provides detailed information on how SPN migrate in the presence or absence of thereelingene. We found that the initial migration (prior to E11.5) of SPN from the neuroepithelium to the ventrolateral spinal cord is similar in both control (wild‐type and heterozygous) andreelermice. However, as development progressed (E12.5–E15.5), SPN in control mice migrated dorsally toward the intermediate lateral spinal cord region, where 80% settled to form the intermediolateral column (IML); the rest migrated medially to locations between the IML and the central canal. Inreeler, 80% of SPN migrated dorsomedially to cluster around the central canal, with the rest distributed between the central canal and the intermediate lateral spinal cord region. The present study also examined the relationship among SPN, Reelin, and radial glial fibers in control andreelermice. Confocal microscopic studies showed that during their initial migration, SPN in both control andreelermice were closely apposed to radial glial fibers in the ventrolateral spinal cord. The majority of SPN in control mice then migrated dorsolaterally, in a direction perpendicular to radial glial fibers, to form the IML. In contrast, the majority of SPN inreelermigrated in the same orientation as radial glial fibers back toward the central canal, instead of migrating dorsolaterally to form the IML. A possible explanation for these results is that Reelin acts to prevent SPN from back‐migration on radial glial fibers toward the central canal. J. Comp. Neurol. 460:94–105, 2003. © 2003 Wiley‐Liss, Inc.