Characterization, Expression, and Interaction Analyses of OsMORF Gene Family in Rice
Characterization, Expression, and Interaction Analyses of OsMORF Gene Family in Rice
复制标题
DOI：
10.3390/genes10090694
复制
发表时间：
2019-09
期刊：
影响因子：
3.5
通讯作者：
Qiang Zhang;Lan Shen;Deyong Ren;Jiang Hu;Guang Chen;Li Zhu;Zhenyu Gao;Guangheng Zhang;Longbiao Guo;D. Zeng;Q. Qian
中科院分区：
文献类型：
--
作者：
Qiang Zhang;Lan Shen;Deyong Ren;Jiang Hu;Guang Chen;Li Zhu;Zhenyu Gao;Guangheng Zhang;Longbiao Guo;D. Zeng;Q. Qian
多细胞器RNA编辑因子（MORF）基因家族在有花植物细胞器RNA编辑中起着关键作用。MORF基因的表达也受到非生物胁迫的影响。目前已在水稻中鉴定出7个OsMORF基因，但有关OsMORF基因在不同组织中的表达模式、在非生物胁迫下的表达模式以及OsMORF基因与OsMORF基因的互作关系的报道较少。本研究分析了OsMORF家族基因的基因结构。系统发育分析表明，MORF家族成员在不同植物中可分为6个亚群。7个OsMORF基因在叶片中高表达。低温和盐胁迫分别影响6个和7个OsMORF基因的表达。OsMORF-OsMORF相互作用分析表明，OsMORF 1、OsMORF 8a和OsMORF 8b可以相互作用形成同源异构体。此外，五个OsMORF蛋白被证明能够相互作用，如OsMORF 8a和OsMORF 8b分别与OsMORF 1和OsMORF 2b相互作用，形成异聚体。这些结果为进一步研究OsMORF基因的功能提供了信息。
The multiple organellar RNA editing factors (MORF) gene family plays a key role in organelle RNA editing in flowering plants. MORF genes expressions are also affected by abiotic stress. Although seven OsMORF genes have been identified in rice, few reports have been published on their expression patterns in different tissues and under abiotic stress, and OsMORF–OsMORF interactions. In this study, we analyzed the gene structure of OsMORF family genes. The MORF family members were divided into six subgroups in different plants based on phylogenetic analysis. Seven OsMORF genes were highly expressed in leaves. Six and seven OsMORF genes expressions were affected by cold and salt stresses, respectively. OsMORF–OsMORF interaction analysis indicated that OsMORF1, OsMORF8a, and OsMORF8b could each interact with themselves to form homomers. Moreover, five OsMORF proteins were shown to be able to interact with each other, such as OsMORF8a and OsMORF8b interacting with OsMORF1 and OsMORF2b, respectively, to form heteromers. These results provide information for further study of OsMORF gene function.