Redesigning Bacillus thuringiensis Cry1Aa toxin into a mosquito toxin
Redesigning Bacillus thuringiensis Cry1Aa toxin into a mosquito toxin
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DOI：
10.1093/protein/gzj009
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发表时间：
2006-03-01
影响因子：
2.4
通讯作者：
Dean, DH
中科院分区：
文献类型：
--
作者：
Liu, XS;Dean, DH
苏云金芽孢杆菌晶体蛋白 Cry1Aa 通常对毛毛虫幼虫具有选择性活性。通过合理设计，通过对结构域II的环残基进行选择性删除和替换，引入了对淡色库蚊的毒性（μg/ml）。对天然目标天蛾的毒性也同时被消除。将替代蚊子毒性成功移植到原始鳞翅目 Cry1Aa 毒素上，证明了通过重塑具有所需特异性的受体结合区域，将所需毒性设计和工程化到常见支架的任何毒素中的可能性。
The Bacillus thuringiensis crystal protein Cry1Aa is normally selectively active to caterpillar larvae. Through rational design, toxicity (mu g/ml) to the mosquito Culex pipiens was introduced by selected deletions and substitutions of the loop residues of domain II. Toxicity to its natural target Manduca sexta was concomitantly abolished. The successful grafting of the alternate mosquito toxicity onto the original lepidopteran Cry1Aa toxin demonstrates the possibility of designing and engineering a desired toxicity into any toxin of a common scaffold by reshaping the receptor binding region with desired specificities.