Enhancement of triterpenoid saponins biosynthesis in Panax notoginseng cells by co-overexpressions of 3-hydroxy-3-methylglutaryl CoA reductase and squalene synthase genes
Enhancement of triterpenoid saponins biosynthesis in Panax notoginseng cells by co-overexpressions of 3-hydroxy-3-methylglutaryl CoA reductase and squalene synthase genes
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DOI：
10.1016/j.bej.2017.03.001
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发表时间：
2017-06-15
影响因子：
3.9
通讯作者：
Chen, Chao-Yin
中科院分区：
文献类型：
--
作者：
Deng, Bing;Zhang, Ping;Chen, Chao-Yin
三七三萜皂苷类化合物广泛应用于心脑血管疾病的治疗，需求量日益增加。本研究将三萜皂苷生物合成途径中的关键酶基因3-羟基-3-甲基戊二酰辅酶A还原酶（PnHMGR）和角鲨烯合成酶（PnSS）导入三七细胞，以提高三七细胞中三萜皂苷的产量。PnHMGR单独过表达或PnSS和PnHMGR共过表达均能显著提高总皂苷的积累。结果表明，PnSS和PnHMGR共过表达细胞株的总皂苷产量最高，分别是对照和过表达细胞株的3倍和1.5倍。此外，PnHMGR-PnSS转基因细胞系中6种主要单体皂苷（Re、Rb 1、Rg 1、Rh 1、Rh 2和F1）和植物甾醇的含量也有所增加。本研究结果为提高药用植物中三萜皂苷的含量提供了一种有效的基因工程手段。(C)2017 Elsevier B. V.版权所有。
Triterpene saponins from Panax notoginseng are widely used in the treatment of cardio-cerebrovascular diseases with increasing demand. Herein, key enzyme genes 3-hydroxy-3-methylglutaryl CoA reductase (PnHMGR) and squalene synthase (PnSS) involved in the triterpene saponins biosynthetic pathway were introduced into Panaxnotoginseng cells to enhance triterpene saponins productions. Overexpression of PnHMGR alone or co-overexpression of PnSS and PnHMGR could remarkably enhance the accumulation of total saponins compared with the control. The highest yield of total saponins was got from the co-overexpression of PnSS and PnHMGR cell line, which was 3-fold and 1.5-fold higher than those in control and the cell line overexpressed PnHMGR alone, respectively. In addition, the contents of six major monomer saponins (Re, Rb1, Rg1, Rh1, Rh2, and F1) and phytosterols were also increased in PnHMGR-PnSS transgenic cell lines. Our results provide a useful genetic engineering strategy to improve the contents of triterpene saponins in many medicinal plants. (C) 2017 Elsevier B.V. All rights reserved.