Development of an animal-borne blood sample collection device and its deployment for the determination of cardiovascular and stress hormones in phocid seals
Development of an animal-borne blood sample collection device and its deployment for the determination of cardiovascular and stress hormones in phocid seals
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动物源性血液样本采集装置的开发及其用于测定海豹心血管和应激激素的应用
DOI：
10.1152/ajpregu.00211.2016
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Ailsa Hall
中科院分区：
文献类型：
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作者：
Yoshio Takei;Ippei Suzuki (equal contribution);Marty K. Wong;Ryan Milne;Simon Moss;Katsufumi Sato;Ailsa Hall
研制了一种具有数据记录功能的海豹动物血样采样器，采集了两种不同条件下的血样，进行内分泌/生化指标的比较。采样器可以通过预设的静液压力、加速度(下降或上升)、温度和时间触发，也可以通过光线手动触发。抽样是可靠的，39/50(78%)的尝试成功地采集了血液样本。管子中的液体对下一次血液样本的污染率为1%，这是在事先将管子清理到废旧注射器之后。在圈养海豹(Phoca Vitulina)中，自动采血方法的压力比直接采血要小，这一点从压力荷尔蒙水平较低(促肾上腺皮质激素和皮质醇分别为P<；0.05和P=0.078)中得到了证明。高效液相分析表明，皮质醇和皮质醇都在海豹血中循环。用该采样器比较了灰海豹(Halichoerus Grypus)在陆地和水中采集的样品中心血管激素、心钠素(ANP)、血管紧张素Ⅱ(AVP)和血管紧张素Ⅱ(Ang II)的水平。高效液相色谱分析确定[Met12]ANP(1-28)和不同形式的血管紧张素(Ang II、III和IV)在海豹血中循环。尽管水浸泡对陆生哺乳动物血浆心血管激素水平有显著影响，但海豹的心钠素水平仅有升高趋势(P=0.069)，血管紧张素转换酶水平呈下降趋势(P=0.074)。这些结果表明，在食肉动物进化到半水生生活方式的过程中，食肉海豹的心血管调节可能经历了适应。
An animal-borne blood sampler with data-logging functions was developed for phocid seals, which collected two blood samples for the comparison of endocrinological/biochemical parameters under two different conditions. The sampler can be triggered by preset hydrostatic pressure, acceleration (descending or ascending), temperature, and time, and also manually by light. The sampling was reliable with 39/50 (78%) successful attempts to collect blood samples. Contamination of fluids in the tubing to the next blood sample was <1%, following the prior clearance of the tubing to a waste syringe. In captive harbor seals (Phoca vitulina), the automated blood-sampling method was less stressful than direct blood withdrawal, as evidenced by lower levels of stress hormones (P< 0.05 for ACTH andP= 0.078 for cortisol). HPLC analyses showed that both cortisol and cortisone were circulating in seal blood. Using the sampler, plasma levels of cardiovascular hormones, atrial natriuretic peptide (ANP), AVP, and ANG II were compared in grey seals (Halichoerus grypus), between samples collected when the animals were on land and in the water. HPLC analyses determined that [Met12] ANP (1–28) and various forms of angiotensins (ANG II, III, and IV) were circulating in seal blood. Although water immersion profoundly changes the plasma levels of cardiovascular hormones in terrestrial mammals, there were only tendencies toward an increase in ANP (P= 0.069) and a decrease in AVP (P= 0.074) in the seals. These results suggest that cardiovascular regulation in phocid seals may have undergone adaptation during evolution of the carnivore to a semiaquatic lifestyle.