Conversion of Ser to Thr residues at the sperm combining-site of mZP3 does not affect sperm receptor activity.
Conversion of Ser to Thr residues at the sperm combining-site of mZP3 does not affect sperm receptor activity.
复制标题
mZP3 精子结合位点的 Ser 残基转化为 Thr 残基不会影响精子受体活性。
DOI：
10.1016/s0006-291x(03)00044-5
复制
发表时间：
2003
影响因子：
3.1
通讯作者：
Wassarman,PaulM
中科院分区：
文献类型：
--
作者：
Williams,Zev;Litscher,EvelineS;Wassarman,PaulM
Mammalian eggs are surrounded by a thick extracellular coat, the zona pellucida, that is composed of three glycoproteins, called ZP1-3. Sperm recognize and bind to O-linked oligosaccharides attached to Ser-332 and Ser-334 at the sperm combining-site of mouse ZP3 (mZP3). Mutation of either of these Ser residues to a small aliphatic amino acid results in the loss of sperm binding to mZP3 in vitro. Here, we converted both Ser-332 and Ser-334 to Thr residues by site-directed mutagenesis. Recombinant mutant glycoprotein made by stably transfected EC cells was purified and then assayed for its ability to inhibit binding of sperm to ovulated eggs in vitro. Results of these experiments suggest that Thr residues can replace the two evolutionarily conserved Ser residues as acceptors for essential O-linked oligosaccharides at the sperm combining-site of mZP3 without affecting the glycoprotein’s sperm receptor activity.
