Airway responses to aerosolized methacholine in the cat. Effects of partial or complete vagosympathectomy.
Airway responses to aerosolized methacholine in the cat. Effects of partial or complete vagosympathectomy.
复制标题
猫气道对雾化乙酰甲胆碱的反应。
DOI：
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
J. Martin
中科院分区：
文献类型：
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作者：
T. Bai;P. Macklem;J. Martin
为了探讨气道神经支配作为气道对吸入性胆碱能激动剂反应性的决定因素的作用，我们研究了部分或完全切断颈迷走交感干对氯醛糖-氨基甲酸乙酯麻醉的乙酰甲胆碱诱导的支气管收缩幅度的影响，机械通风猫。初始浓度-对乙酰甲胆碱的反应曲线被构建以确定引起平均肺阻力（RL）增加两倍至三倍所需的浓度。使用该浓度，然后在4组猫中以30分钟间隔进行3次连续激发（C1、C2和C3）。对照激发（C1）后，将颈迷走交感神经干的副交感神经（n = 9）、交感神经（n = 6）或两个分支（n = 4）切片。假手术猫（n = 7）显示RL可重现性增加（C1，Δ RL = 15.1 +/- 2.7 cm H2O X 1（-1）X s; C2，Δ RL = 15.7 +/- 3.0; C3，Δ RL = 13.1 +/- 2.9;平均值+/- 1 SE）。副交感神经切除术后，C3后的反应性增加（C1，Δ RL = 15.0 +/- 4.3; C2，Δ RL = 16.4 +/- 6.4; C3，Δ RL = 29.9 +/- 11.6; p <0.01）。交感神经切除术和迷走交感神经切除术没有显着改变反应性。结果表明，迷走神经介导的抑制作用调节支气管反应雾化乙酰甲胆碱在猫。
To explore the role of airway innervation as a determinant of airway responsiveness to inhaled cholinergic agonists, we studied the effects of partial or complete section of the cervical vagosympathetic trunk on the magnitude of methacholine-induced bronchoconstriction in the chloralose-urethane-anesthetized, mechanically ventilated cat. An initial concentration-response curve to methacholine was constructed to identify the concentration required to cause a twofold to threefold rise in mean pulmonary resistance (RL). Using this concentration, 3 consecutive challenges (C1, C2, and C3) were then performed at 30-min intervals in 4 groups of cats. After a control challenge (C1), the parasympathetic (n = 9), sympathetic (n = 6), or both branches (n = 4) of the cervical vagosympathetic trunks were sectioned. Sham cats (n = 7) showed reproducible increases in RL (C1, delta RL = 15.1 +/- 2.7 cm H2O X 1(-1) X s; C2, delta RL = 15.7 +/- 3.0; C3, delta RL = 13.1 +/- 2.9; mean +/- 1 SE). After parasympathectomy there was an increase in responsiveness after C3 (C1, delta RL = 15.0 +/- 4.3; C2, delta RL = 16.4 +/- 6.4; C3, delta RL = 29.9 +/- 11.6; p less than 0.01). Sympathectomy and vagosympathectomy did not significantly alter responsiveness. The results suggest that vagally mediated inhibitory influences modulate the bronchial response to aerosolized methacholine in the cat.