Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications.
Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications.
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DOI：
10.1021/acsmaterialsau.1c00056
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发表时间：
2022-03-09
期刊：
影响因子：
--
通讯作者：
D'Sa, Raechelle A
中科院分区：
文献类型：
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作者：
Li, Man;Aveyard, Jenny;Doherty, Kyle G;Deller, Robert C;Williams, Rachel L;Kolegraff, Keli N;Kaye, Stephen B;D'Sa, Raechelle A
不愈合和慢性伤口代表了患者生活质量的主要问题，并且对医疗保健系统具有成本影响。阻止伤口愈合的病理生理机制通常是多因素的，并且与患者的整体健康和营养、伤口床的血管分布以及共存的感染/定植有关。细菌感染是一个主要的问题，可以停止伤口，导致它成为慢性。成功的伤口愈合通常取决于数周或数月的抗菌治疗，但鉴于多重耐药细菌的增加，这是有问题的。因此，迫切需要抗生素的替代品来帮助慢性甚至急性感染伤口的愈合。一氧化氮（NO）通过多种机制杀死细菌，因此，细菌没有显示出对作为治疗剂的NO产生抗性的趋势，因此可以是抗生素治疗的良好替代方案。在本文中，我们报告了一氧化氮释放静电纺丝膜的发展，由聚己内酯（PCL）/明胶共混物和优化，以减少细菌感染。膜中的NO有效载荷与共混物中的胺的数量（以及因此明胶的量）直接相关。更高的NO有效载荷对应于更高程度的抗微生物功效。电纺膜没有观察到细胞毒性，体外伤口闭合试验证明在16小时内闭合。这里呈现的结果清楚地表明，这些释放NO的电纺膜由于其抗微生物活性和生物相容性而具有作为伤口敷料的显著前景。
Nonhealing and chronic wounds represent a major problem for the quality of life of patients and have cost implications for healthcare systems. The pathophysiological mechanisms that prevent wound healing are usually multifactorial and relate to patient overall health and nutrition, vascularity of the wound bed, and coexisting infection/colonization. Bacterial infections are one of the predominant issues that can stall a wound, causing it to become chronic. Successful wound healing often depends on weeks or months of antimicrobial therapy, but this is problematic given the rise in multidrug-resistant bacteria. As such, alternatives to antibiotics are desperately needed to aid the healing of chronic, and even acutely infected wounds. Nitric oxide (NO) kills bacteria through a variety of mechanisms, and thus, bacteria have shown no tendency to develop resistance to NO as a therapeutic agent and therefore can be a good alternative to antibiotic therapy. In this paper, we report on the development of NO-releasing electrospun membranes fabricated from polycaprolactone (PCL)/gelatin blends and optimized to reduce bacterial infection. The NO payload in the membranes was directly related to the number of amines (and hence the amount of gelatin) in the blend. Higher NO payloads corresponded with a higher degree of antimicrobial efficacy. No cytotoxicity was observed for electrospun membranes, and an in vitro wound closure assay demonstrated closure within 16 h. The results presented here clearly indicate that these NO-releasing electrospun membranes hold significant promise as wound dressings due to their antimicrobial activity and biocompatibility.