NDC10 - A GENE INVOLVED IN CHROMOSOME SEGREGATION IN SACCHAROMYCES-CEREVISIAE
NDC10 - A GENE INVOLVED IN CHROMOSOME SEGREGATION IN SACCHAROMYCES-CEREVISIAE
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DOI：
10.1083/jcb.121.3.503
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发表时间：
1993-05-01
影响因子：
7.8
通讯作者：
KILMARTIN, JV
中科院分区：
文献类型：
--
作者：
GOH, PY;KILMARTIN, JV
从芽殖酵母温度敏感突变体库中，通过抗微管蛋白染色，分离到一个突变体ndc 10 -1。NDC 10 -1在染色体分离方面有缺陷，因为染色体保留在后期纺锤体的一极。这产生了一个多倍体细胞和一个非倍体细胞，每个细胞都含有一个纺锤体极体（SPB）。NDC 10被克隆和测序，并且与CBF 2相同（Jiang，W.，J. Lechner J. Carbon。1993. 121：513-519），其是着丝粒DNA结合复合物的110-kD组分（Lechner，J.，和J. Carbon。1991. Cell. 64：717-725）。NDC 10是一个必需基因。Ndc 10 p的抗体标记的SPB区域在几乎所有的细胞检查，包括非有丝分裂细胞。在一些可能处于中期的短纺锤体细胞中，也观察到沿纺锤体沿着染色。ndc 10 -1的染色模式和表型与作为动粒蛋白的Cbf 2 p/Ndc 10 p一致，并提供了其在染色体附着到纺锤体中的作用的体内证据。
A mutant, ndc10-1, was isolated by anti-tubulin staining of temperature-sensitive mutant banks of budding yeast. ndc10-1 has a defect in chromosome segregation since chromosomes remain at one pole of the anaphase spindle. This produces one polyploid cell and one aploid cell, each containing a spindle pole body (SPB). NDC10 was cloned and sequenced and is identical to CBF2 (Jiang, W., J. Lechner. and J. Carbon. 1993. J. Cell Biol. 121:513-519) which is the 110-kD component of a centromere DNA binding complex (Lechner, J., and J. Carbon. 1991. Cell. 64:717-725). NDC10 is an essential gene. Antibodies to Ndc10p labeled the SPB region in nearly all the cells examined including nonmitotic cells. In some cells with short spindles which may be in metaphase, staining was also observed along the spindle. The staining pattern and the phenotype of ndc10-1 are consistent with Cbf2p/Ndc10p being a kinetochore protein, and provide in vivo evidence for its role in the attachment of chromosomes to the spindle.