The F-box protein HAWAIIAN SKIRT is required for mimicry target-induced microRNA degradation in Arabidopsis
The F-box protein HAWAIIAN SKIRT is required for mimicry target-induced microRNA degradation in Arabidopsis
复制标题
F-box 蛋白 HAWAIIAN SKIRT 是拟南芥中拟态靶标诱导的 microRNA 降解所必需的。
DOI：
10.1111/jipb.12761
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发表时间：
2019-11-01
影响因子：
11.4
通讯作者：
Ren, Guodong
中科院分区：
文献类型：
--
作者：
Mei, Jun;Jiang, Ning;Ren, Guodong
模拟靶向microRNA降解在真核生物中广泛存在且高度保守。然而，对其作用机制知之甚少。在这封信中，通过使用STTM 160（miR 160的靶模拟物）作为报告基因，我们表明夏威夷裙（HWS）功能障碍抑制了STTM 160的多效性表型。小RNA测序和北方印迹分析表明，HWS只影响一个子集的microRNA。有趣的是，当HWS受损时，我们确定了miR 160/miR 399及其模拟靶标在AGO 1复合物内的稳定共存，这表明HWS在清除与模拟靶标相关的RNA诱导的沉默复合物中可能起作用。
Mimicry target-directed microRNA degradation is widespread and highly conserved among eukaryotes. However, little is known about its mechanism of action. In this letter, by using STTM160 (target mimic of miR160) as a reporter, we show that dysfunction of HAWAIIAN SKIRT (HWS) suppresses the pleiotropic phenotype of STTM160. Small RNA sequencing and Northern blot analyses suggested that HWS only affects a subset of microRNAs. Intriguingly, we identified a stable coexistence of miR160/miR399 and their mimicry targets within the AGO1 complex when HWS is compromised, pointing to a possible role of HWS in the clearance of RNA-induced silencing complexes associated with mimicry target.