Prohibitin as an oxidative stress biomarker in the eye.
Prohibitin as an oxidative stress biomarker in the eye.
复制标题
在眼中禁止作为氧化应激生物标志物。
DOI：
10.1016/j.ijbiomac.2010.08.018
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发表时间：
2010-12-01
影响因子：
8.2
通讯作者：
Jahng WJ
中科院分区：
文献类型：
--
作者：
Lee H;Arnouk H;Sripathi S;Chen P;Zhang R;Bartoli M;Hunt RC;Hrushesky WJ;Chung H;Lee SH;Jahng WJ
在视网膜和视网膜色素上皮细胞（RPE）氧化应激下的生物标志物蛋白的鉴定可能意味着在分子水平上对视网膜变性的信号转导机制的新见解。将来自恒定光照下的体内小鼠模型和体外氧化应激模型的蛋白质组学数据与正常条件下的对照进行比较。我们的蛋白质组学研究表明，prohibitin参与视网膜和RPE的氧化应激信号。使用2D电泳、免疫组织化学、蛋白质印迹和质谱分析研究视网膜和RPE中抑制素的身份。与凋亡标志物以及线粒体和细胞核之间的易位的表达水平的比较意味着抑制素的调节是在氧化应激下的RPE和视网膜中的早期信号事件。免疫组织化学分析的小鼠老年人和糖尿病的眼睛进一步表明，在RPE/视网膜的抑制蛋白的调节与衰老和糖尿病诱导的氧化应激。我们的蛋白质组学方法表明，在RPE和视网膜中的抑制素可能是一个新的生物标志物蛋白的氧化应激在衰老和糖尿病。
Identification of biomarker proteins in the retina and the retinal pigment epithelium (RPE) under oxidative stress may imply new insights into signaling mechanisms of retinal degeneration at the molecular level. Proteomic data from an in vivo mice model in constant light and an in vitro oxidative stress model are compared to controls under normal conditions. Our proteomic study shows that prohibitin is involved in oxidative stress signaling in the retina and RPE. The identity of prohibitin in the retina and the RPE was studied using 2D electrophoresis, immunohistochemistry, western blot, and mass spectrometry analysis. Comparison of expression levels with apoptotic markers as well as translocation between mitochondria and the nucleus imply that the regulation of prohibitin is an early signaling event in the RPE and retina under oxidative stress. Immunohistochemical analysis of murine aged and diabetic eyes further suggests that the regulation of prohibitin in the RPE/retina is related to aging- and diabetes-induced oxidative stress. Our proteomic approach implies that prohibitin in the RPE and the retina could be a new biomarker protein of oxidative stress in aging and diabetes.
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影响因子：
4.8
作者：
Jiang, XJ;Wang, XD
通讯作者：
Wang, XD
影响因子：
4.1
作者：
Lee, HC;Yin, PH;Wei, YH
通讯作者：
Wei, YH
影响因子：
2.9
作者：
Xue, Linlong;Jahng, Wan Jin;Rando, Robert R.
通讯作者：
Rando, Robert R.
DOI：
10.1073/pnas.94.2.514
发表时间：
1997-01-21
影响因子：
11.1
作者：
Yakes, FM;VanHouten, B
通讯作者：
VanHouten, B
影响因子：
3.7
作者：
Coates, PJ;Nenutil, R;Wright, EG
通讯作者：
Wright, EG