Orange peel extract reduces the inflammatory state of skeletal muscle after downhill running via an increase in IL-1RA
Orange peel extract reduces the inflammatory state of skeletal muscle after downhill running via an increase in IL-1RA
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DOI：
10.1093/bbb/zbab049
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发表时间：
2021-03-19
影响因子：
1.6
通讯作者：
Sato, Ryuichiro
中科院分区：
文献类型：
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作者：
Suzuki, Toshihide;Shimizu, Makoto;Sato, Ryuichiro
据报道，橙皮提取物(OPE)和OPE中的4种主要多甲氧基黄酮(PMF)对下坡跑(DR)引起的骨骼肌炎症有保护作用。然而，其机制还没有被很好地理解。我们研究了OPE和PMF化合物提高抗炎细胞因子水平的可能性。OPE给药后1h和8h，大鼠血浆IL-1RA水平明显升高。诺比莱汀可诱导C2C12肌管分泌IL-1RA。在DR后骨骼肌炎症状态下，OPE可降低核因子-kappa-轻链活化B细胞增强剂(nf-kappa-轻链增强子B)的表达、核因子-kappaB-DNA结合和单核细胞趋化蛋白-1m RNA水平，但这些作用均被静脉注射IL-1RA中和抗体所抵消。这些结果表明，OPE通过增加IL-1RA来减轻DR后骨骼肌炎症状态，IL-1受体信号转导在DR后骨骼肌炎症反应中起重要作用。
It has been reported that orange peel extract (OPE) and the 4 major polymethoxyflavones (PMFs) in OPE have a protective effect against downhill running (DR)-induced skeletal muscle inflammation. However, the mechanism is not well understood. We investigated the potential of OPE and PMF compounds for increasing anti-inflammatory cytokine levels. The plasma interleukin-1 receptor antagonist (IL-1RA) level was increased 1 and 8 h after OPE administration in rats. Nobiletin induced the secretion of IL-1RA from C2C12 myotubes. In the inflammatory state of skeletal muscle after DR, OPE administration reduced nuclear factor kappa-light-chain enhancer of activated B cells (NF-kappa B) expression, NF-kappa B-DNA binding, and monocyte chemotactic protein-1 mRNA levels, but these effects were all abrogated by the intravenous administration of IL-1RA neutralizing antibody. These results indicated that OPE reduces skeletal muscle inflammatory state after DR via an increase in IL-1RA, and that IL-1 receptor signaling is important for skeletal muscle inflammation after DR.