BRINE SHRIMP - A CONVENIENT GENERAL BIOASSAY FOR ACTIVE-PLANT CONSTITUENTS
BRINE SHRIMP - A CONVENIENT GENERAL BIOASSAY FOR ACTIVE-PLANT CONSTITUENTS
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DOI：
10.1055/s-2007-971236
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发表时间：
1982-01-01
期刊：
影响因子：
2.7
通讯作者：
MCLAUGHLIN, JL
中科院分区：
文献类型：
--
作者：
MEYER, BN;FERRIGNI, NR;MCLAUGHLIN, JL
提出了一种利用卤虾（A. salina Leach）作为天然产物研究的简单生物测定方法。该程序确定LC50[平均致死浓度]值。G /ml盐水培养基中的活性化合物和提取物。广泛的已知活性化合物的活性表现为对虾的毒性。对大麻科41种植物种子提取物的9KB和9PS细胞毒性进行了比较。该方法是一种快速、可靠、廉价、方便的内部通用生物测定工具。
A method, utilizing brine shrimp (A. salina Leach), is proposed as a simple bioassay for natural product research. The procedure determines LC50 [mean lethal concentration] values in .mu.g/ml of active compounds and extracts in the brine medium. Activities of a broad range of known active compounds are manifested as toxicity to the shrimp. Screening results with seed extracts of 41 spp. of Euphorbiaceae were compared with 9KB and 9PS cytotoxicities. The method is rapid, reliable, inexpensive and convenient as an in-house general bioassay tool.