CLEC4C gene expression can be used to quantify circulating plasmacytoid dendritic cells
CLEC4C gene expression can be used to quantify circulating plasmacytoid dendritic cells
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DOI：
10.1016/j.jim.2018.11.001
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发表时间：
2019-01-01
影响因子：
2.2
通讯作者：
Upham, John W.
中科院分区：
文献类型：
--
作者：
Murray, Liisa;Xi, Yang;Upham, John W.
浆细胞样树突状细胞（Plasmacytoid dendritic cells，pDC）是一种重要的I型干扰素产生者，在病毒感染的第一道宿主防御中发挥重要作用。pDC数量和功能的异常与几种健康状况有关。定量pDC对于理解病毒感染和其他疾病中的pDC相关免疫应答是重要的，然而，目前使用流式细胞术定量pDC的方法在涉及多个中心的大型队列研究中的实用性有限，其中流式细胞术的使用有限。我们推断，检测pDC标志物C型凝集素结构域家族4成员C（CLEC4C，也称为CD303和BDCA 2）与pDC排他性白细胞免疫球蛋白样受体A4（LILRA 4，也称为CD85 g和ILT7）的组合的基因表达可能提供可应用于多中心研究的更实用的方法。我们的结果显示，通过表面染色测量的pDC数量与全血中CLEC4C基因表达之间存在中度相关性（rho = 0.39，P = 0.037），并且全血和外周血单核细胞中CLEC4C基因表达之间存在高度相关性（rho = 0.79，P <0.001）。LILRA 4基因表达没有提供额外有用的信息。我们的研究结果表明，测量CLEC4C基因表达可以提供一种替代方法，用于定量人样本中的pDC数量。
Plasmacytoid dendritic cells (pDC) are an important type I interferon producer that play an important role in the first line of host defence during viral infection. Abnormalities in pDC numbers and function have been associated with several health conditions. Quantifying pDC is important for understanding pDC related immune responses in viral infections and other diseases, however the current methods for quantifying pDC using flow cytometry have limited utility in large cohort studies involving multiple centres with limited access to flow cytometry. We reasoned that examining gene expression of the pDC marker C-type lectin domain family 4 member C (CLEC4C, also known as CD303 and BDCA2) in combination with pDC exclusive leukocyte immunoglobulin like receptor A4 (LILRA4, also known as CD85g and ILT7) might provide a more practical method that could be applied to multi-centre studies. Our results show a moderate correlation between pDC numbers measured by surface staining and CLEC4C gene expression in whole blood (rho = 0.39, P = .037, as well as a high correlation between CLEC4C gene expression in whole blood and peripheral blood mononuclear cells (rho = 0.79, P < .001). LILRA4 gene expression did not provide additional useful information. Our results indicate that measuring CLEC4C gene expression can provide an alternative method for quantifying pDC numbers in human samples.