Ratiometric Electrogenerated Chemiluminescence Cytosensor Based on Conducting Polymer Hydrogel Loaded with Internal Standard Molecules
Ratiometric Electrogenerated Chemiluminescence Cytosensor Based on Conducting Polymer Hydrogel Loaded with Internal Standard Molecules
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DOI：
10.1021/acs.analchem.8b04116
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发表时间：
2019-01-01
影响因子：
7.4
通讯作者：
Luo, Xiliang
中科院分区：
文献类型：
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作者：
Ding, Caifeng;Li, Yunxia;Luo, Xiliang
以CdTe量子点和鲁米诺为双发光体，构建了一个灵敏、可靠的双峰电致发光（ECL）体系。用癌细胞的适体（CdTe-Apt 2）标记的CdTe量子点用作检测信号，而鲁米诺分子用作内标。电沉积在电极表面的聚苯胺基导电聚合物水凝胶（CPH）有效地改善了传感界面的生物相容性和导电性。此外，电子转移可能是更容易当鲁米诺和共反应物过硫酸钾（K2 S2 O 8）固定在CPH中相比，在溶液中。癌细胞被另一种通过Au-S键连接到固定在CPH中的Au纳米颗粒的适体捕获到电极表面。在所开发的双峰ECL系统中，使用内标方法通过比较双峰ECL信号与目标分析物的灵敏度的差异来定量癌细胞。ECL策略的内标法可以通过两个发射光谱的自校准消除体系中的干扰，从而在复杂环境中提供非常准确的检测结果。在优化条件下，基于Δ ECLCdTe/AECL（鲁米诺）对100-6500个细胞mL（-1）内的癌细胞浓度的曲线图，发现线性关系。所开发的具有内标的比率型ECL细胞传感器可以显著提高复杂生物介质中细胞测定的准确性和可靠性，在医疗监测和临床诊断中显示出有前途的应用。
A sensitive and reliable bimodal electro-chemiluminescent (ECL) system based on CdTe quantum dots (QDs) and luminol as double luminophores is constructed. CdTe QDs tagged with the aptamer (CdTe-Apt 2) of cancer cells are used as the detection signals, while luminol molecules are used as internal standards. The electrodeposited polyaniline-based conducting polymer hydrogel (CPH) on the electrode surfaces improves the biocompatibility and conductivity of the sensing interfaces effectively. Furthermore, electron transfer is probably much easier when luminol and coreactant potassium persulfate (K2S2O8) are immobilized in the CPH in comparison to that in solution. Cancer cells are captured to the electrode surface by another aptamer linked to the Au nanoparticles immobilized in the CPH through Au-S bonds. In the developed bimodal ECL system, an internal standard method is used to quantify cancer cells by comparing the differences in sensitivity of the double-peak ECL signals with that of target analytes. The internal standard method of ECL strategy can provide very accurate detection results in a complex environment because interferences in the system can be eliminated through the self-calibration of two emission spectra. A linear relation is found on the basis of a plot of the Delta ECLCdTe/AECL(luminol) against the concentration of cancer cells within 100-6500 cells mL(-1) under optimized conditions. The developed ratiometric ECL cytosensor with internal standard can significantly improve the accuracy and reliability of cell assays in complex biological media, demonstrating promising applications in healthcare monitoring and clinical diagnostics.