Production of itaconic acid by Pseudozyma antarctica NRRL Y-7808 under nitrogen-limited growth conditions
Production of itaconic acid by Pseudozyma antarctica NRRL Y-7808 under nitrogen-limited growth conditions
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DOI：
10.1016/j.enzmictec.2006.01.005
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发表时间：
2006-08-02
影响因子：
3.4
通讯作者：
Kuo, Tsung Min
中科院分区：
文献类型：
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作者：
Levinson, William E.;Kurtzman, Cletus P.;Kuo, Tsung Min
在氮限制生长条件下，发现Pseudozyma anapartica NRRLY-7808从葡萄糖和其它糖产生衣草酸。筛选出的其他Pseudozyma菌株，包括另一株R. anastritica，不产生这种产物;因此，衣草酸的产生不是该属的共同特征。葡萄糖和果糖是最有效利用的底物，其次是蔗糖和麦芽糖;乳糖和甘油是最差的底物。摇瓶实验中的最大产率为37.5%（从80 g/L葡萄糖约30 g/L亚硝酸）。在摇瓶培养中，当C/N比为116时，最大产酶速率为248 mg L-1 h（-1），而当C/N比为175时，产酶速率和产量的最佳组合分别为230 mg L-1 h（-1）和29%.一个搅拌罐反应器的过程中的研究，导致在20%的产率和体积生产率为110毫克L-1小时（-1）。爱思唯尔公司出版
Pseudozyma antarctica NRRLY-7808 was found to produce itaconic acid from glucose and other sugars under nitrogen-limited growth conditions. Other Pseudozyma strains screened, including a second strain of R antarctica, did not produce this product; therefore itaconic acid production is not a common trait of the genus. Glucose and fructose were the most efficiently utilized substrates, followed by sucrose and maltose; lactose and glycerol were the poorest substrates. The maximum yield in flask experiments was 37.5% (approximately 30 g/L itaconic acid from 80 g/L glucose). The maximum rate of production in flask cultures was 248 mg L-1 h(-1) at a C/N ratio of 116, while the best combination of rate and yield was produced at a C/N ratio of 175 (230 mg L-1 h(-1) and 29%, respectively). A stirred-tank reactor process study resulted in a 20% yield and volumetric production rate of 110 mg L-1 h(-1). Published by Elsevier Inc.