Characterization of G2L3 (GAS2-like 3), a new microtubule- and actin-binding protein related to spectraplakins.
Characterization of G2L3 (GAS2-like 3), a new microtubule- and actin-binding protein related to spectraplakins.
复制标题
DOI：
10.1074/jbc.m111.242263
复制
发表时间：
2011-07-15
期刊：
影响因子：
--
通讯作者：
Ballestrem C
中科院分区：
文献类型：
--
作者：
Stroud MJ;Kammerer RA;Ballestrem C
微管（MT）和肌动蛋白细胞骨架是细胞完整性的基础，因为它们控制着许多细胞活动，包括细胞分裂、生长、极化和迁移。参与介导MT和肌动蛋白细胞骨架之间的串扰的蛋白质是许多细胞过程的关键，并发挥重要的生理作用。我们确定了MT-actin交联蛋白GAS2家族的新成员，命名为G2L3 （GAS2-like 3）。我们发现GAS2-like 3在整个进化过程中广泛保守，并且在人体组织中普遍表达。GAS2-like 3分别通过其单独的钙钙蛋白同源3型结构域和C端与丝状肌动蛋白和MTs相互作用。有趣的是，假定的与mt结合的gas2相关结构域的作用是调节GAS2-like 3与丝状肌动蛋白和mt的结合，这与在相关蛋白中发现的gas2相关结构域相反，gas2相关结构域的功能是作为mt结合结构域。此外，我们发现微管蛋白乙酰化驱动GAS2-like 3定位到mt，并可能为GAS2-like 3的功能作用提供新的见解。
The microtubule (MT) and actin cytoskeletons are fundamental to cell integrity, because they control a host of cellular activities, including cell division, growth, polarization, and migration. Proteins involved in mediating the cross-talk between MT and actin cytoskeletons are key to many cellular processes and play important physiological roles. We identified a new member of the GAS2 family of MT-actin cross-linking proteins, named G2L3 (GAS2-like 3). We show that GAS2-like 3 is widely conserved throughout evolution and is ubiquitously expressed in human tissues. GAS2-like 3 interacts with filamentous actin and MTs via its single calponin homology type 3 domain and C terminus, respectively. Interestingly, the role of the putative MT-binding GAS2-related domain is to modulate the binding of GAS2-like 3 to both filamentous actin and MTs. This is in contrast to GAS2-related domains found in related proteins, where it functions as a MT-binding domain. Furthermore, we show that tubulin acetylation drives GAS2-like 3 localization to MTs and may provide functional insights into the role of GAS2-like 3.