Up-regulation of miR-9 target CBX7 to regulate invasion ability of bladder transitional cell carcinoma.
Up-regulation of miR-9 target CBX7 to regulate invasion ability of bladder transitional cell carcinoma.
复制标题
DOI：
10.12659/msm.893232
复制
发表时间：
2015-01-18
期刊：
影响因子：
--
通讯作者：
Tong X
中科院分区：
文献类型：
--
作者：
Xie D;Shang C;Zhang H;Guo Y;Tong X
膀胱尿路上皮癌是我国最常见的泌尿生殖系统恶性肿瘤。本研究旨在探讨miR-9是否通过下调CBX 7来调节人膀胱移行细胞癌细胞的侵袭能力。采用实时荧光定量PCR检测膀胱移行细胞癌（TCC）和正常膀胱移行细胞（NBTC）中miR-9的表达。利用生物信息学软件对miR-9的潜在靶基因进行预测。将pre-miR-9转染T24细胞，Western blot检测CBX 7蛋白的表达。通过荧光素酶活性测定验证CBX 7是miR-9的直接特异基因。将pcDNA-CBX 7转染T24细胞，检测CBX 7基因的表达。transwell法检测CBX 7过表达的T24细胞的侵袭能力。与NBTC组织相比，TCC组织中miR-9的表达显著增加。TargetScan和PicTar软件预测CBX 7基因是miR-9的靶基因。pre-miR-9可上调miR-9的表达，下调CBX 7蛋白的表达。荧光素酶活性检测证实CBX 7基因是miR-9的直接特异性靶基因。pcDNA-CBX 7转染可上调CBX 7蛋白的表达，CBX 7过表达的T24细胞侵袭能力明显下降。miR-9的异常表达促进了T24细胞的侵袭，部分是通过直接下调膀胱移行细胞癌中CBX 7蛋白的表达。这种miRNA特征为TCC提供了一个新的潜在治疗靶点。
Bladder urothelial carcinoma is the most common genitourinary system cancer in China. The objective of this study was to investigate whether the miR-9 can regulate the invasion ability of human bladder transitional cell carcinoma cells by down-regulation of CBX7. The expression of miR-9 was detected by quantitative real-time PCR in bladder transitional cell carcinomas (TCC) and normal bladder transitional cell (NBTC) samples. Bioinformatics software was used to predict some potential target genes of miR-9. T24 cells were transfected with pre-miR-9, and the CBX7 protein expression was detected by Western blot. Luciferase activities assay was selected to verify that CBX7 was a direct and specific gene of miR-9. T24 cells were transfected with pcDNA-CBX7, and the expression of CBX7 gene was detected. Then, the transwell assay was used to detect the invasion ability of T24 cells with CBX7 over-expression. The expression of miR-9 increased significantly in human TCC specimens compared to that in NBTC specimens. TargetScan and PicTar software programs predicted CBX7 gene was a target gene of miR-9. The pre-miR-9 could up-regulate the miR-9 expression and down-regulate CBX7 protein expression. The luciferase activities assay verified that CBX7 gene was a direct and specific target gene of miR-9. The pcDNA-CBX7 transfection could up-regulate the CBX7 protein expression, and the invasion ability of T24 cells with CBX7 over-expression decreased significantly. Aberrantly expressed miR-9 contributes to T24 cells invasion, partly through directly down-regulating CBX7 protein expression in TCC. This miRNA signature offers a new potential therapeutic target for TCC.