The heat-inducible zebrafish hsp70 gene is expressed during normal lens development under non-stress conditions
The heat-inducible zebrafish hsp70 gene is expressed during normal lens development under non-stress conditions
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DOI：
10.1016/s0925-4773(01)00652-9
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发表时间：
2002-03-01
影响因子：
2.6
通讯作者：
Krone, PH
中科院分区：
文献类型：
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作者：
Blechinger, SR;Evans, TG;Krone, PH
在本研究中，我们发现，斑马鱼的应激诱导热休克蛋白70基因是强烈的，特异性表达在正常的透镜形成后28至38小时受精，随后下调2天的年龄。在其他胚胎组织中仅零星观察到弱的组成性hsp70 mRNA信号。类似地，携带与cGFP连接的hsp70启动子的1.5 kb片段的转基因鱼仅在透镜中显示荧光。与此相反，内源hsp70基因和转基因强烈表达整个胚胎热休克后在相同的发育阶段。(C)2002爱思唯尔科学爱尔兰有限公司保留所有权利。
In the present study, we show that the stress-inducible hsp70 gene in zebrafish is strongly and specifically expressed during normal lens formation from 28 to 38 hours post-fertilization, and is subsequently downregulated by 2 days of age. Only weak constitutive hsp70 mRNA signal was sporadically observed in other embryonic tissues. Similarly, transgenic fish carrying a 1.5 kb fragment of the hsp70 promoter linked to cGFP exhibited fluorescence only in the lens. In contrast, both the endogenous hsp70 gene and the transgene were strongly expressed throughout the embryo following heat shock at the same developmental stages. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.