Gibberellin stimulates regrowth after defoliation of sheepgrass (Leymus chinensis) by regulating expression of fructan-related genes
Gibberellin stimulates regrowth after defoliation of sheepgrass (Leymus chinensis) by regulating expression of fructan-related genes
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赤霉素通过调节果聚糖相关基因的表达来刺激羊草（羊草）落叶后的再生
DOI：
10.1007/s10265-016-0832-1
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发表时间：
2016-09-01
影响因子：
2.8
通讯作者：
Chen, Shuangyan
中科院分区：
文献类型：
--
作者：
Cai, Yueyue;Shao, Linhui;Chen, Shuangyan
赤霉素（Gibberellins, GAs）影响牧草生长发育；然而，GAs如何调节果聚糖（许多谷物中重要的多糖储备）的代谢和饲料植物脱叶后的再生，在很大程度上是未知的。探讨脱叶羊草（Leymus chinensis, Trin.）的响应机制。[Tzvel]对赤霉素进行了研究，我们用ga3和/或多氯布唑（paclobutrazol，一种赤霉素生物合成抑制剂）喷洒落叶羊草，分析了赤霉素代谢、赤霉素信号转导和果聚糖代谢相关基因的生长特性、碳水化合物含量和转录水平。结果表明：与对照相比，外源ga3喷施对脱叶羊草叶片和节间伸长有促进作用，而PAC喷施对叶片和节间伸长有抑制作用。在脱叶羊草上喷洒ga3也通过延长果聚糖的利用时间而改变了果聚糖的含量。在转录水平上，外源ga3增加了鞘中GA代谢相关基因的转录水平。综上所述，我们的研究结果表明，外源ga3通过调节GA和果聚糖相关基因，并通过促进内源GA合成、果聚糖代谢和信号传导来促进脱落羊草的再生。
Gibberellins (GAs) affect forage growth and development; however, it is largely unknown how GAs regulate the metabolism of fructan (an important polysaccharide reserve in many cereals) and the regrowth of forage plants after defoliation. To explore the mechanism of the responses of defoliated sheepgrass [Leymus chinensis(Trin.) Tzvel] to GA, we sprayed defoliated sheepgrass with GA3and/or paclobutrazol (PAC; an inhibitor of GA biosynthesis) and analyzed the growth characteristics, carbohydrate contents, and transcript levels of genes related to GA metabolism, GA signal transduction, and fructan metabolism. The results showed that spraying exogenous GA3onto defoliated sheepgrass promoted leaf and internode elongation, while spraying with PAC inhibited leaf and internode elongation, compared with the control. Spraying GA3onto defoliated sheepgrass also altered the fructan content by extending the period of fructan utilization. At the transcriptional level, exogenous GA3increased the transcript levels of genes related to GA metabolism in the sheath. Taken together, our results suggest that exogenous GA3stimulates the regrowth of defoliated sheepgrass regrowth by regulating GA and fructan-related genes, and by promoting endogenous GA synthesis, fructan metabolism, and signaling.