TIME-DEPENDENT LOSS OF INVASIVE ABILITY OF PLASMODIUM-BERGHEI MEROZOITES INVITRO
TIME-DEPENDENT LOSS OF INVASIVE ABILITY OF PLASMODIUM-BERGHEI MEROZOITES INVITRO
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DOI：
10.2307/3280000
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发表时间：
1977-01-01
影响因子：
1.3
通讯作者：
MCALISTER, RO
中科院分区：
文献类型：
--
作者：
MCALISTER, RO
采用补体介导的免疫裂解法，在体外和体内研究了伯氏疟原虫裂殖子从寄生的小鼠红细胞中人工去除后的体内侵袭能力。时程实验表明，裂解制剂含有2种成分，有助于寄生虫在小鼠中的感染性。一种组分，推测是从成熟的裂殖子感染的细胞释放的游离裂殖子，在1-2 ℃下随着时间的推移迅速失去感染性。第二种次要成分在该温度下似乎具有更大的稳定性，并且可以解释为含有成熟裂殖体的完整寄生细胞，其在体外未被补体溶解，但在体内被受体血浆补体溶解。将寄生的细胞悬浮在等渗稀释剂中并以中等速度离心，基本上去除了通过免疫溶血从给定的感染血液样品中不可分离的侵入性游离裂殖子的数量。当在等渗培养基中悬浮并在体外保持在1-2 ℃时，裂殖子或者被包含在成熟的疟原虫感染的细胞的范围内，或者被人工从宿主细胞中去除，在体内迅速丧失侵入易感红细胞的能力。
The invasive ability of P. berghei merozoites in vivo was studied following their artificial removal from parasitized mouse red cells using complement-mediated immune lysis in vitro and in vivo. Time-course experiments revealed that lysed preparations contained 2 components contributing to the parasites'' infectivity in mice. One component, presumed to be free merozoites released from mature schizont-infected cells, rapidly lost infectivity with time at 1-2 C. A 2nd minor component appeared to have more stability at this temperature, and could be accounted for as intact parasitized cells containing mature schizonts not lysed by the complement in vitro, but lysed by the recipients'' plasma complement in vivo. Suspension of parasitized cells in an isotonic diluent and centrifugation at moderate speeds substantially removes the number of invasive free merozoites insolable from a given sample of infected blood by immune hemolysis. Merozoites either contained within the confines of mature schizont-infected cells or artificially removed from host cells rapidly lose the ability to invade susceptible erythrocytes in vivo when suspended in an isotonic medium and held at 1-2 C in vitro.