A cynomolgus monkey model of temporal lobe epilepsy
A cynomolgus monkey model of temporal lobe epilepsy
复制标题
食蟹猴颞叶癫痫模型
DOI：
10.1016/j.brainresbull.2018.11.001
复制
发表时间：
2019-01-01
影响因子：
3.8
通讯作者：
Xu, Qi
中科院分区：
文献类型：
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作者：
Chen, Ting;Deng, Yu;Xu, Qi
颞叶癫痫（TLE）伴海马硬化是最常见的耐药性癫痫类型。非人灵长类动物是研究TLE致病机制的有吸引力的模型，目的是开发新的药物和干预措施。在这项研究中，我们开发和测试了食蟹猴（食蟹猴）TLE模型。共5只食蟹猴接受每周3 -4次的单侧海马注射红藻氨酸（KA）以诱导重复性急性癫痫发作。通过视频和脑电图（EEG）监测动物，以评估KA诱导的急性癫痫发作和随后的自发复发性癫痫样放电（SRED）。在急性癫痫发作期间，EEG记录显示KA注射同侧颞叶出现全身性棘波放电。三个月后，我们检测到丰富的发作间期癫痫样放电（IEDs）在戊巴比妥诱导麻醉。此外，两只猴子表现出同步癫痫样放电伴随着症状模仿失神发作。在所有猴中均未观察到明显的惊厥症状。总的来说，我们的数据表明通过单侧海马注射KA成功开发了食蟹猴TLE模型。
Temporal lobe epilepsy (TLE) with hippocampal sclerosis is the most common type of drug-resistant epilepsy. Non-human primates are attractive models for studying the pathogenic mechanisms of TLE, with the goal of developing new drugs and interventions. In this study, we developed and tested a Cynomolgus monkey (Macaca fascicularis) model of TLE. A total of 5 Cynomolgus monkeys received3-4 weekly unilateral hippocampal injections of kainic acid (KA) to induce repetitive acute seizures. Animals were monitored via video and electroencephalography (EEG) to assess KA-induced acute seizures and subsequent spontaneous recurrent epileptiform discharges (SREDs). During acute seizures, EEG recording showed bursts of generalized spike discharges arising from the temporal lobe ipsilateral to the KA injection. Three months later, we detected abundant interictal epileptiform discharges (IEDs) during pentobarbital induced anesthesia. Furthermore, two monkeys exhibited synchronized epileptiform discharges accompanied by symptoms mimicking absence seizures. No obvious convulsive symptoms were observed in any monkeys. Overall, our data indicate successful development of a Cynomolgus monkey model of TLE via unilateral hippocampal injection of KA.