Spatiotemporal Distribution of Chondroitin Sulfate Proteoglycans in the Developing Mouse Retina and Optic Nerve
Spatiotemporal Distribution of Chondroitin Sulfate Proteoglycans in the Developing Mouse Retina and Optic Nerve
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DOI：
10.1292/jvms.10-0201
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发表时间：
2011-01-01
影响因子：
1.2
通讯作者：
Uehara, Masato
中科院分区：
文献类型：
--
作者：
Ali, Safwat Ali Mohamed;Hosaka, Yoshinao Z.;Uehara, Masato
本研究的目的是通过免疫组织化学方法测定硫酸软骨素蛋白多糖在产前和产后小鼠视网膜和视神经中的分布。胚胎日(E) 18时，视网膜和视神经中检测到4-硫酸软骨素（C45）、6-硫酸软骨素（C6S）和biglycan。然而，聚集蛋白在视网膜中可见，而在视神经中未见。出生后第7天，在视神经、内核层和神经节细胞层可见明显的聚集蛋白和大聚蛋白，在外视网膜可见弥漫性分布。C4S弥漫性分布于视网膜和视神经，而C6S主要局限于感光层和视神经鞘。P42时，biglycan在视网膜和视神经内呈弥漫性分布，神经纤维丰富层呈强烈染色。内丛状层聚集蛋白染色较弱，外核层、内核层、外丛状层和神经节细胞层聚集蛋白密度较高。C4S和C6S均见于视神经和视网膜，但C6S在感光层表现出较强的免疫染色。这些蛋白多糖在视网膜和视神经发育过程中的时间分布表明它们在视网膜和视神经的发育和维持中可能具有独特或重叠的作用。
The aim of the present study was to determine the distribution of chondroitin sulfate proteoglycans in the mouse retina and optic nerve of the prenatal and postnatal mouse by immunohistochemistry. At embryonic day (E) 18, chondroitin-4-sulfate (C45), chondroitin-6-sulfate (C6S) and biglycan were detected in the retina and optic nerve. However, aggrecan was seen in the retina but not in the optic nerve. At postnatal day (P) 7, aggrecan and biglycan were clearly observed in the optic nerve, inner nuclear layer and ganglion cell layer and diffuse in the outer retina. C4S diffusely distributed in the retina and optic nerve, but C6S was mainly confined to the photoreceptor layer and optic nerve sheath. At P42, biglycan showed diffuse distribution in the retina and optic nerve with intense staining in nerve-fiber rich layers. Aggrecan showed weak staining at the inner plexiform layer with higher density in the outer and inner nuclear layers, outer plexiform layer and ganglion cell layer. Both C4S and C6S were detected in the optic nerve and retina, but C6S showed strong immunostaining in the photoreceptor layer. The distributions of these proteoglycans with respect of time course during development of the retina and optic nerve suggest that they may have unique or overlapping roles in development and maintenance of the retina and optic nerve.