Impregnation of β-tricalcium phosphate robocast scaffolds by in situ polymerization
Impregnation of β-tricalcium phosphate robocast scaffolds by in situ polymerization
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DOI：
10.1002/jbm.a.34609
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发表时间：
2013-11-01
影响因子：
4.9
通讯作者：
Miranda, Pedro
中科院分区：
文献类型：
--
作者：
Martinez-Vazquez, Francisco J.;Perera, Fidel H.;Miranda, Pedro
将ε-己内酯（epsilon-CL）和L-丙交酯（LLA）开环聚合于机械流延成型的磷酸三钙（-TCP）支架上。从-TCP商业粉末制备的浓缩胶体油墨用于制造由互穿棒的3D网格组成的多孔结构。分别使用脂肪酶和辛酸亚锡作为催化剂，在陶瓷结构内原位聚合ε-CL和LLA。结果表明，在任一情况下，陶瓷网内的大孔和陶瓷棒内的微孔都充满了聚合物。通过原位聚合（ISP）浸渍的支架的机械性能比裸结构的机械性能显著增加，表现出与通过其他更具侵略性的浸渍方法（例如熔融浸渍（MI））获得的机械性能相似的值。使用酶催化剂的ISP需要降低的加工温度，这可以促进生长因子和其他药物掺入聚合物组合物中，从而增强复合支架的生物活性。这些结果的影响骨组织工程应用支架的机械和生物性能的优化进行了讨论。(c)2013 Wiley Periodicals，Inc. J Biomed Mater Res Part A：101A：3086-3096，2013.
Ring-opening polymerization of epsilon-caprolactone (epsilon-CL) and L-lactide (LLA) was performed to impregnate -tricalcium phosphate (-TCP) scaffolds fabricated by robocasting. Concentrated colloidal inks prepared from -TCP commercial powders were used to fabricate porous structures consisting of a 3D mesh of interpenetrating rods. epsilon-CL and LLA were in situ polymerized within the ceramic structure by using a lipase and stannous octanoate, respectively, as catalysts. The results show that both the macropores inside the ceramic mesh and the micropores within the ceramic rods are full of polymer in either case. The mechanical properties of scaffolds impregnated by in situ polymerization (ISP) are significantly increased over those of the bare structures, exhibiting similar values than those obtained by other, more aggressive, impregnation methods such as melt-immersion (MI). ISP using enzymatic catalysts requires a reduced processing temperature which could facilitate the incorporation of growth factors and other drugs into the polymer composition, thus enhancing the bioactivity of the composite scaffold. The implications of these results for the optimization of the mechanical and biological performance of scaffolds for bone tissue engineering applications are discussed. (c) 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 3086-3096, 2013.