Testosterone in the fetal rat testis.
Testosterone in the fetal rat testis.
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胎鼠睾丸中的睾酮。
DOI：
10.1093/biolreprod/8.5.560
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发表时间：
1973
影响因子：
3.6
通讯作者：
K. Eik‐Nes
中科院分区：
文献类型：
--
作者：
D. Warren;G. C. Haltmeyer;K. Eik‐Nes
胎儿大鼠的睾丸早在子宫内 15.5 天时就具有将放射性醋酸盐转化为放射性睾酮所需的酶。因此，胎儿睾丸能够在内生殖器的性别分化期间重新形成睾酮。在胎鼠的睾丸组织中测量睾酮。数值范围从妊娠 15.5 天时低至 0.09 ng/mg，到妊娠 18.5 天时低至 2.76 ng/mg。胎龄 18.5 天时的急剧上升与形态学证据相一致，即此时沃利夫管稳定需要相对大量的睾酮。胎儿睾丸也与[‘4C]乙酸盐或[‘H]孕烯醇酮一起孵育。虽然这两种底物在妊娠 15.5 天和 16.5 天时都转化为放射性睾酮，但在胎龄 14.5 天时，任何一种底物都没有明显的这种转化。然而，在所有三个时间段的孵化过程中都可以测量非放射性睾酮。在妊娠 15 至 20 天时，在用放射性醋酸盐或孕烯醇酮孵育胎儿卵巢时，未检测到放射性或非放射性睾酮。类固醇雄激素的影响
Testes of fetal rats possess the enzymes necessary for converting radioactive acetate to radioactive testosterone as early as 15.5 days of intrauterine life. The fetal testis is, therefore, capable of formation do novo of testosterone through the period of sexual differentiation of the internal genitalia. Testosterone was measured in testicular tissue of fetal rats. Values ranged from as low as 0.09 ng/mg at 15.5 days of gestation to 2.76 ng/mg at 18.5 days of gestation. The sharp rise at 18.5 days of fetal age coincides with morphological evidence that relatively large amounts of testosterone are necessary for Wolifian duct stabilization at this time. Fetal testes were also incubated with [‘4C] acetate or [‘H] pregnenolone. Whereas conversion of both substrates to radioactive testosterone occurred at 15.5 and 16.5 days of gestation, no such conversion was evident at 14.5 days of fetal age with either substrate. Nonradioactive testosterone was, however, measurable in incubation from all three time periods. Neither radioactive nor nonradioactive testosterone was detectable in incubations of fetal ovaries with radioactive acetate or pregnenolone at 15 to 20 days of gestation. The influence of steroid androgens on