RESPECTIVE ROLES OF LAMININ AND FIBRONECTIN IN ADHESION OF HUMAN CARCINOMA AND SARCOMA-CELLS
RESPECTIVE ROLES OF LAMININ AND FIBRONECTIN IN ADHESION OF HUMAN CARCINOMA AND SARCOMA-CELLS
复制标题
DOI：
10.1038/289304a0
复制
发表时间：
1981-01-01
期刊：
影响因子：
64.8
通讯作者：
GOSPODAROWICZ, D
中科院分区：
文献类型：
--
作者：
VLODAVSKY, I;GOSPODAROWICZ, D
尤文S肉瘤细胞和结肠癌细胞不能附着在塑料和涂有I、III和IV型胶原的培养皿上，但能迅速附着于培养的角膜内皮细胞产生的细胞外基质上。虽然癌细胞需要细胞外基质，并且不附着于纤维连接蛋白，但肉瘤细胞在这两种底物上的黏附和扁平几乎一样好。不同的黏附蛋白可能介导肉瘤和癌症来源的细胞与细胞外基质的黏附，最有可能的是纤维连接蛋白和层粘连蛋白。纤维连接蛋白可促进成纤维细胞与IV型胶原的黏附，但不能刺激表皮细胞黏附，并可介导肉瘤细胞的黏附。层粘连蛋白局限于基底膜的透明板层区域，并定位于细胞黏附部位。对表皮细胞体外附着的研究表明，它对上皮细胞具有粘附性，因此它对癌细胞的作用可能与纤维连接蛋白对肉瘤细胞的作用相同。培养的血管内皮细胞分泌纤维连接蛋白和层粘连蛋白到细胞外基质和培养液中，塑料培养皿预先暴露于这种条件培养液中，可诱导人结肠癌和尤文S肉瘤细胞的附着和扁平化。层粘连蛋白介导前者的附着和铺展，而纤维粘连蛋白负责后者的附着和扁平。
Ewing''s sarcoma cells and colon carcinoma cells failed to attach to plastic and to dishes coated with collagen types I, III and IV but adhered rapidly to extracellular matrix (ECM) produced by cultured corneal endothelial cells. Although carcinoma cells required ECM and did not adhere to fibronectin, the sarcoma cells adhered and flattened almost equally well on either substrate. Different adhesive proteins may mediate the attachment of sarcoma- and carcinoma-derived cells to extracellular matrices, the most likely being fibronectin and laminin. Fibronectin stimulates the adhesion of fibroblasts, but not epidermal cells, to collagen type IV and could mediate the attachment of sarcoma cells. Laminin is confined to the lamina lucida region of basement membranes and has been localized to cellular adhesion sites. Studies of the attachment of epidermal cells in vitro suggest that it is adhesive for epithelial cells and so could have the same role for carcinoma cells as that played by fibronectin for sarcoma cells. Cultured vascular endothelial cells secrete fibronectin and laminin into the ECM and the culture medium and pre-exposure of plastic dishes to such conditioned medium induces the attachment and flattening of human colon carcinoma and Ewing''s sarcoma cells. While laminin mediates the attachment and spreading of the former, fibronectin is responsible for the attachment and flattening of the latter.