Dopamine modulates metabolic rate and temperature sensitivity in Drosophila melanogaster.
Dopamine modulates metabolic rate and temperature sensitivity in Drosophila melanogaster.
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DOI：
10.1371/journal.pone.0031513
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Kume K
中科院分区：
文献类型：
--
作者：
Ueno T;Tomita J;Kume S;Kume K
居家动物，如哺乳动物，通过产热和散热来维持体温，而变温动物，如昆虫，通过迁移到它们喜欢的温度环境来实现。已知儿茶酚胺调节哺乳动物的产热和代谢率，但其在其他动物中的作用知之甚少。果蝇（Drosophilamelanogaster）一直被用作温度偏好行为遗传研究的模型系统。在这里，我们证明代谢率和温度敏感性的一些温度敏感性行为的调节多巴胺在果蝇。像dTrpA 1和shits这样的温度敏感分子会诱导温度依赖性的行为变化，而在多巴胺转运蛋白缺陷突变体fumin中诱导这些变化的温度会降低。该突变体还表现出对较低温度的偏好。多巴胺神经元中多巴胺转运蛋白的遗传恢复挽救了这种热恐惧表型。用多巴胺生物合成抑制剂（3-碘-L-酪氨酸）喂养的苍蝇，减少多巴胺信号，表现出对较高温度的偏好。此外，我们发现，代谢率上调的富明突变体。综上所述，多巴胺在果蝇行为变化的温度敏感性和代谢率调节中具有功能，以及其先前报道的唤醒/睡眠调节功能。
Homeothermal animals, such as mammals, maintain their body temperature by heat generation and heat dissipation, while poikilothermal animals, such as insects, accomplish it by relocating to an environment of their favored temperature. Catecholamines are known to regulate thermogenesis and metabolic rate in mammals, but their roles in other animals are poorly understood. The fruit fly, Drosophila melanogaster, has been used as a model system for the genetic studies of temperature preference behavior. Here, we demonstrate that metabolic rate and temperature sensitivity of some temperature sensitive behaviors are regulated by dopamine in Drosophila. Temperature-sensitive molecules like dTrpA1 and shi ts induce temperature-dependent behavioral changes, and the temperature at which the changes are induced were lowered in the dopamine transporter-defective mutant, fumin. The mutant also displays a preference for lower temperatures. This thermophobic phenotype was rescued by the genetic recovery of the dopamine transporter in dopamine neurons. Flies fed with a dopamine biosynthesis inhibitor (3-iodo-L-tyrosine), which diminishes dopamine signaling, exhibited preference for a higher temperature. Furthermore, we found that the metabolic rate is up-regulated in the fumin mutant. Taken together, dopamine has functions in the temperature sensitivity of behavioral changes and metabolic rate regulation in Drosophila, as well as its previously reported functions in arousal/sleep regulation.
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影响因子：
2.2
作者：
Goto, SG;Kimura, MT
通讯作者：
Kimura, MT
影响因子：
64.8
作者：
Hong, Sung-Tae;Bang, Sunhoe;Kim, Jaeseob
通讯作者：
Kim, Jaeseob
DOI：
10.1098/rstb.2002.1074
发表时间：
2002-07-29
影响因子：
6.3
作者：
Bale, JS
通讯作者：
Bale, JS
影响因子：
5.3
作者：
Kume, K;Kume, S;Jackson, FR
通讯作者：
Jackson, FR
影响因子：
3.7
作者：
Kong EC;Woo K;Li H;Lebestky T;Mayer N;Sniffen MR;Heberlein U;Bainton RJ;Hirsh J;Wolf FW
通讯作者：
Wolf FW