Differences in protein changes between stress-induced premature senescence and replicative senescence states
Differences in protein changes between stress-induced premature senescence and replicative senescence states
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DOI：
10.1002/elps.201300086
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发表时间：
2013-08-01
期刊：
影响因子：
2.9
通讯作者：
Karsani, Saiful Anuar
中科院分区：
文献类型：
--
作者：
Aan, Goon Jo;Hairi, Haryati Ahmad;Karsani, Saiful Anuar
复制性衰老和应激诱导的早衰（SIPS）细胞具有某些共同特征。然而，这些细胞在蛋白质水平上是否不同尚不清楚。因此，本研究利用蛋白质组学来鉴定复制性衰老和SIPS细胞与正常细胞相比蛋白质组的差异。复制性衰老是由正常细胞在培养物中连续传代诱导的。通过暴露于20 μ M亚细胞毒性浓度的H2 O2两周建立SIPS。2DE后，蛋白质谱进行了比较，并通过MALDI-TOF MS鉴定丰度变化的蛋白质点。然后进行定量实时RT-PCR以评估所选改变蛋白质的转录表达。共有24个和10个蛋白质被发现在复制衰老和SIPS细胞，分别改变了丰富的年轻细胞相比。定量RT-PCR显示，9个基因显示相同的方向的变化中观察到的蛋白质组学分析。在复制性衰老和SIPS细胞中变化的蛋白质之间几乎没有重叠，这表明尽管SIPS和复制性衰老细胞都具有细胞衰老的标志，但它们在丰度变化的蛋白质方面是不同的。
Replicative senescence and stress-induced premature senescence (SIPS) cells are known to share certain traits. However, whether these cells are different at the protein level is unclear. Thus, this study has utilized proteomics to identify differences in the proteomes of replicative senescence and SIPS cells compared to normal cells. Replicative senescence was induced by serial passage of normal cells in culture. SIPS was established by exposure to H2O2 at a subcytotoxic concentration of 20 mu M for two weeks. Following 2DE, protein profiles were compared and protein spots that changed in abundance were identified by MALDI-TOF MS. Quantitative real-time RT-PCR was then performed to evaluate the transcript expression of selected altered proteins. A total of 24 and 10 proteins were found to have changed in abundance in replicative senescence and SIPS cells, respectively, when compared to young cells. Quantitative RT-PCR revealed that nine genes showed the same direction of change as observed in the proteomics analysis. Very little overlap was observed between proteins that changed in replicative senescence and SIPS cells, suggesting that although both SIPS and replicative senescence cells share hallmarks of cellular senescence, they were different in terms of proteins that changed in abundance.