Metabolite profiling reveals the involvement of aberrant metabolic changes in Gentiana triflora seed showing poor germination
Metabolite profiling reveals the involvement of aberrant metabolic changes in Gentiana triflora seed showing poor germination
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代谢分析揭示了三花龙胆种子发芽不良的异常代谢变化
DOI：
10.1080/14620316.2015.1117229
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发表时间：
2016
影响因子：
1.9
通讯作者：
and Nishihara M.
中科院分区：
文献类型：
--
作者：
Takahashi H;Fujita K;Yoshida C;and Nishihara M.
龙胆是一种高山植物，也是我国特有的两种植物：三花龙胆和龙胆。Scabra，在日本作为观赏植物种植。由于大多数日本品种都是F1，种子发芽率是维持这些品种的一个重要因素。然而，不同品种的发芽率往往不同。发芽率低是龙胆生产的一个严重问题，尽管它们的发芽期和生长速度几乎没有区别。在这项研究中，我们比较了三个品种的种子发芽率。特里弗罗拉。‘Majel’(MJ)和‘Iwate’(IW)的种子萌发率较高，而‘Iwate-yumminori’(Ym)的种子发芽率较低。我们利用这些品种进行了有针对性的代谢组分析，以阐明种子萌发受到抑制的潜在原因。多变量分析表明，YM种子中特定氨基酸的异常积累和能量代谢产物的减少。此外，YM种子的蛋白质浓度和蛋白水解酶活性低于MJ种子。这些结果表明，能量消耗是种子萌发率下降的主要原因，这种能量消耗抑制了种子萌发所涉及的过程，包括萌发所需蛋白质的新合成。
Gentians are alpine plants and two endemic species,Gentiana trifloraandG. scabra, are cultivated as ornamentals in Japan. Because most of the Japanese cultivars are F1hybrids, seed germination rate is an important factor for maintaining these cultivars. However, germination rates frequently differ among cultivars. Poor germination is a severe problem for gentian production, although there is almost no difference in their germination period and growth rate. In this study, we compared seed germination rates among three cultivars ofG. triflora. A high rate of seed germination was observed in ‘Majel’ (MJ) and ‘Iwate’ (IW), but a low rate was recorded in ‘Iwate-Yumeminori’ (YM). We conducted a targeted metabolome analysis using these cultivars to elucidate the potential cause of inhibited seed germination. Multivariate analysis revealed that the aberrant accumulation of specific amino acids and a decrease in energy metabolites were observed in YM seed. Furthermore, protein concentrations and proteolytic activities in YM seed were lower than in MJ seed. These results imply that energy depletion was the main reason for the decreased rate of germination and that this depletion inhibited processes involved in seed germination, includingde novosynthesis of the proteins necessary for germination.