Cell aggregation: a mechanism of pathogenic Leptospira to survive in fresh water.
Cell aggregation: a mechanism of pathogenic Leptospira to survive in fresh water.
复制标题
DOI：
10.2436/im.v7i1.9442
复制
发表时间：
2004-03
期刊：
影响因子：
--
通讯作者：
G. Trueba;S. Zapata;Kleber P Madrid;Paul A. Cullen;D. Haake
中科院分区：
文献类型：
--
作者：
G. Trueba;S. Zapata;Kleber P Madrid;Paul A. Cullen;D. Haake
致病性钩端螺旋体在哺乳动物宿主以外的潮湿环境中存活，促进了钩端螺旋体病的传播。本研究对犬型问号钩端螺旋体在水环境和缺乏营养条件下的生存机制进行了研究。在蒸馏水中，钩端螺旋体能够保持运动110天（pH 7.2）。然而，当在由蒸馏水和0.5%纯化琼脂糖（pH 7.2）组成的半固体培养基中孵育时，它们存活347天。在这种粘性环境中，观察到活螺旋体的聚集体。抗生素（如四环素和氨苄青霉素）和营养素都不能抑制钩端螺旋体聚集。免疫印迹分析表明，在水中孵育的细胞下调LipL31，内膜蛋白的表达，但保留其他膜蛋白的表达。这些研究为致病性钩端螺旋体在自然水环境中长时间生存的机制提供了见解，这是钩端螺旋体生命周期的关键阶段。
Transmission of leptospirosis is facilitated by the survival of pathogenic leptospires in moist environments outside their mammalian host. In the present study, the survival mechanisms of Leptospira interrogans serovar Canicola in aqueous conditions and lack of nutrients were investigated. In distilled water, leptospires were able to remain motile for 110 days (pH 7.2). However, when incubated in a semi-solid medium composed of distilled water and 0.5% purified agarose (pH 7.2), they survived 347 days. In this viscous environment, aggregates of live spirochetes were observed. Neither antibiotics (e.g. tetracycline and ampicillin) nor nutrients inhibited leptospiral aggregation. Immunoblot analysis suggested that cells incubated in water down-regulate the expression of LipL31, an inner-membrane protein, but retain expression of other membrane proteins. These studies provide insights into the mechanisms by which pathogenic Leptospira survives for prolonged periods of time in natural aqueous environments, a key stage in the leptospiral lifecycle.