Transcription activation at class II CAP-dependent promoters: Two interactions between CAP and RNA polymerase
Transcription activation at class II CAP-dependent promoters: Two interactions between CAP and RNA polymerase
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DOI：
10.1016/s0092-8674(00)81806-1
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发表时间：
1996-12-13
期刊：
影响因子：
64.5
通讯作者：
Ebright, RH
中科院分区：
文献类型：
--
作者：
Niu, W;Kim, Y;Ebright, RH
在II类分解代谢物激活蛋白（CAP）依赖性启动子处，CAP激活从与RNA聚合酶的DNA位点重叠的DNA位点的转录。我们发现，在II类CAP依赖性启动子的转录激活不仅需要先前表征的CAP激活区和RNA聚合酶亚基C-末端结构域之间的相互作用，而且还需要第二个启动子类特异性CAP激活区和RNA聚合酶亚基N-末端结构域之间的相互作用。我们进一步表明，这两种相互作用影响转录起始的不同步骤。II类CAP依赖性启动子的转录激活为理解激活子如何与转录机制进行多重相互作用提供了一个范例，每个相互作用负责特定的机制后果。
At Class II catabolite activator protein (CAP)-dependent promoters, CAP activates transcription from a DNA site overlapping the DNA site for RNA polymerase. We show that transcription activation at Class II CAP-dependent promoters requires not only the previously characterized interaction between an activating region of CAP and the RNA polymerase a subunit C-terminal domain, but also an interaction between a second, promoter-class-specific activating region of CAP and the RNA polymerase a subunit N-terminal domain. We further show that the two interactions affect different steps in transcription initiation. Transcription activation at Class II CAP-dependent promoters provides a paradigm for understanding how an activator can make multiple interactions with the transcription machinery, each interaction being responsible for a specific mechanistic consequence.