Structure of the VAP-peptide (BmACP-6.7) gene in the silkworm, Bombyx mori and a possible regulation of its expression by BmFTZ-F1.
Structure of the VAP-peptide (BmACP-6.7) gene in the silkworm, Bombyx mori and a possible regulation of its expression by BmFTZ-F1.
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DOI：
10.1016/s0965-1748(99)00105-8
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发表时间：
2000-02
影响因子：
3.8
通讯作者：
K. Shiomi;T. Niimi;K. Imai;O. Yamashita
中科院分区：
文献类型：
--
作者：
K. Shiomi;T. Niimi;K. Imai;O. Yamashita
VAP 肽 (BmACP-6.7) 是一种疏水性肽，位于家蚕成虫角质层中。我们分离并表征了 VAP-肽基因，作为分析成人终末分化过程的分子机制的有用标记基因。该基因由被一个内含子中断的两个外显子组成。显示 5' 上游启动子区域具有与被家蚕 FTZ–F1 蛋白 (BmFTZ–F1) 识别和结合的顺式元件相似的核苷酸序列。 BmFTZ-F1 基因的表达先于 VAP-肽基因的表达，注射 20-羟基蜕皮激素可抑制这两个基因的表达。体外结合测定表明 BmFTZ-F1 与 VAP-肽基因启动子序列直接相互作用。因此，BmFTZ-F1 被认为是调节 VAP-肽基因进入成年阶段的阶段特异性表达的可能因素。
The VAP–peptide (BmACP–6.7) is a hydrophobic peptide localized in adult cuticle of the silkworm, Bombyx mori. We isolated and characterized the VAP–peptide gene as a useful marker gene to analyze molecular mechanisms of terminal differentiation processes in the adult. The gene is composed of two exons interrupted by one intron. The 5′ upstream promoter region is shown to bear a nucleotide sequence similar to the cis-element that is recognized and bound by the Bombyx mori FTZ–F1 protein (BmFTZ–F1). Expression of the BmFTZ–F1 gene preceded expression of the VAP–peptide gene and injection of 20–hydroxyecdysone suppressed the expression of both genes. An in vitro binding assay indicated direct interaction of BmFTZ–F1 with the VAP–peptide gene promoter sequence. Therefore, BmFTZ–F1 is proposed to be a possible factor regulating the stage- specific expression of the VAP–peptide gene towards adult life.