VIP-LIKE IMMUNOREACTIVE NEURONS AND RETINAL PROJECTIONS IN THE RAT SUPRACHIASMATIC NUCLEUS
VIP-LIKE IMMUNOREACTIVE NEURONS AND RETINAL PROJECTIONS IN THE RAT SUPRACHIASMATIC NUCLEUS
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大鼠视交叉上核中的 VIP 样免疫反应神经元和视网膜投影
DOI：
10.2220/biomedres.8.253
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发表时间：
1987
影响因子：
1.2
通讯作者：
Y. Ibata
中科院分区：
文献类型：
--
作者：
H. Okamura;Yukio Takahashi;H. Terubayashi;S. Hamada;Y. Noboru;Y. Ibata
用辣根过氧化物酶（HRP）组织化学、肽免疫细胞化学、电镜和形态计量学方法研究了大鼠视交叉上核（SCN）内VIP样免疫反应神经元与视网膜投射的相互作用。在SCN腹外侧部的对侧和同侧部分检测到充满HRP的视网膜纤维。血管活性肠肽（VIP），肽组氨酸异亮氨酸酰胺（PHI），和胃泌素释放肽（GRP）的免疫反应神经元表现出相同的分布在SCN的中间和尾部水平的HRP填充的视网膜投影。在免疫电镜中，非免疫反应性神经元的VIP免疫反应性神经元的突触接触。电镜下可见视网膜轴突终末吞噬SCN内的树突棘。免疫细胞化学结合彩色图像分析仪的形态计量学分析显示，双侧眼球摘除后7天，VIP免疫反应阳性斑点数量增加。这些结果有力地表明，视网膜传入和含VIP神经元之间的密切相互作用的SCN。
Interactions of retinal projections and VIP-like immunoreactive neurons in the suprachiasmatic nucleus (SCN) of the rat were investigated by horseradish peroxidase (HRP)-histochemical, peptide immunocytochemical, electron microscopic, and morphometric analyses. HRP-filled retinal fibers were detected in the contralateral and ipsilateral part of the ventrolateral part of SCN. Neurons immunoreactive to vasoactive intestinal peptide (VIP), peptide histidine isoleucine amide (PHI), and gastrin releasing peptide (GRP) showed the same distribution as the HRP-filled retinal projections in the intermediate and caudal level of SCN. In immunoelectron microscopy, non-immunoreactive neuropil elements made synaptic contact with VIP-immunoreactive neurons. Degenerating electron microscopic study showed that retinal axon terminals engulfed dendritic spines in SCN. The technique of immunocytochemistry combined with morphometric analysis by color image analyzer revealed that VIP-immunoreactive dots were increased in number 7 days after bilateral enucleation. These results strongly suggest the close interaction between retinal afferents and VIP-containing neurons in SCN.