Neurotransmitter-specific identification and characterization of neurons in the all-cone retina of Anolis carolinensis, I: Gamma-aminobutyric acid.
Neurotransmitter-specific identification and characterization of neurons in the all-cone retina of Anolis carolinensis, I: Gamma-aminobutyric acid.
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卡罗林安乐蜥全视锥视网膜神经元的神经递质特异性鉴定和表征，I：γ-氨基丁酸。
DOI：
10.1017/s0952523800005617
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发表时间：
1992
影响因子：
1.9
通讯作者：
Ulshafer,RJ
中科院分区：
文献类型：
--
作者：
Sherry,DM;Ulshafer,RJ
利用放射自显影和免疫细胞化学技术，在卡罗莱纳蜥蜴纯锥体视网膜中定位了抑制性氨基酸神经递质γ -氨基丁酸（GABA）。摄取[3H]-GABA标记的水平细胞、无突细胞、神经节细胞层、丛状细胞层和神经纤维层中的大量细胞。内丛状层的标签层呈明显的层叠。GABA免疫反应的模式与[3H]-GABA摄取的模式相似，尽管观察到一些差异，特别是在无毛细胞和神经节细胞的标记上。免疫细胞化学显示内源性GABA储存在一组水平细胞、无突细胞和神经节细胞层细胞中。两个网状层均用GABA抗血清标记。内丛状层（IPL）的标记高度分层，层下a和层下b均存在gaba免疫反应层。鉴定出6种常规放置的gaba免疫反应性无毛细胞亚型和1种移位的无毛细胞亚型。6个常规无毛细胞亚型中有3个为梨形形态，3个为多极形态。同时观察到gaba免疫反应性间质细胞。在一定条件下，GABA抗血清标记锥体。蚀刻树脂消除了锥体标记，这表明锥体中的GABA存在于一个不稳定的池中，不像水平细胞或无毛细胞中的GABA，或者观察到的标记不是由于内源性GABA。锥细胞未表现出[3H]-GABA摄取。
The inhibitory amino-acid neurotransmitter, gamma-aminobutyric acid (GABA), was localized in the pure cone retina of the lizard Anolis carolinensis by autoradiographic and immunocytochemical techniques. Uptake of [3H]-GABA labeled horizontal cells, amacrine cells, numerous cells in the ganglion cell layer, both plexiform layers, and the nerve fiber layer. Label in the inner plexiform layer showed distinct lamination.The pattern of GABA immunoreactivity was similar to the pattern of [3H]-GABA uptake, although some differences, particularly in labeling of amacrine and ganglion cells, were observed. Immunocytochemistry revealed endogenous stores of GABA in a set of horizontal cells, amacrine cells, and cells in the ganglion cell layer. Both plexiform layers were labeled by the GABA antisera. Labeling in the inner plexiform layer (IPL) was highly stratified and GABA-immunoreactive strata were present in both sublaminae a and b. Six subtypes of conventionally placed GABA-immunoreactive amacrine cells and one displaced amacrine cell subtype were identified. Three of the six conventional amacrine cell subtypes were of pyriform morphology and three subtypes were of multipolar morphology. GABA-immunoreactive interstitial cells also were observed.Under certain conditions the GABA antiserum labeled the cones. Etching the resin eliminated cone labeling, suggesting that GABA in the cones is present in a labile pool, unlike GABA in horizontal or amacrine cells, or the observed labeling was not due to endogenous GABA. Cones did not demonstrate [3H]-GABA uptake.