EXTENT OF HEPATECTOMY IN THE RAT - EVALUATION OF BASAL CONDITIONS AND EFFECT OF THERAPY
EXTENT OF HEPATECTOMY IN THE RAT - EVALUATION OF BASAL CONDITIONS AND EFFECT OF THERAPY
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DOI：
10.1159/000129034
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发表时间：
1989-09-01
影响因子：
1.6
通讯作者：
HOUSSIN, D
中科院分区：
文献类型：
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作者：
EMOND, J;CAPRONLAUDEREAU, M;HOUSSIN, D
在本研究中，肝次全切除作为急性肝功能衰竭的大鼠模型进行了评价。体重250-300 g的Sprague-Dawley大鼠在不同的温度和葡萄糖给药基础条件下进行肝切除术。术后在环境温度（25 ℃外部环境）下操作和维持的大鼠出现体温过低，其恢复到正常温度的速率与肝切除的程度和术后葡萄糖的可用性有关。 然而，在保持在环境温度下的组和通过被动外部加温将核心温度保持在37 ℃的组之间没有观察到存活率的显著差异。在术后接受90和95%肝切除术的大鼠中观察到严重的低血糖。20度。饮用水中的葡萄糖使90%肝切除的死亡率从95%降低到40%（P < 0.0001）。随着肝切除率增加到95%，尽管有葡萄糖支持，仍观察到90%的死亡率。移植4次在肝切除术时腹腔内注射107个分离的同系肝细胞，在90%或95%的肝切除术后没有增加存活率;单独或肝细胞移植后，加入睾酮治疗没有改善存活率。在这项研究中，肝切除超过90%是致命的，并没有回应所提供的支持措施。肝细胞移植和睾酮预处理，这两种被认为可以增加再生的疗法，在这种切除模型中对提高生存率无效。
In the present study, subtotal hepatectomy was evaluated as a model of acute liver failure in the rat. Sprague-Dawley rats, weighing 250-300 g, underwent hepatectomy under varying basal conditions of temperature and glucose administration. Rats operated and maintained postoperatively at ambient temperature (25 .degree.C external environment) developed hypothermia with a rate of return to normal temperature which was related to the extent of hepatectomy and the availability of glucose postoperatively. However, no significant difference in survival was observed between groups maintained at ambient temperature and those whose core temperature was maintained at 37 .degree.C by passive external warming. Severe hypoglycemia was observed in rats undergoing 90 and 95% hepatectomy without glucose postoperatively. With 20.degree. glucose available in drinking water the mortality of 90% hepatectomy was reduced from 95 to 40% (p < 0.0001). With increase of the hepatectomy to 95%, 90% mortality was observed despite glucose support. Transplantation of 4 .times. 107 isolated syngeneic hepatocytes intraperitoneally at the time of hepatectomy did not increase survival after 90 or 95% hepatectomy; addition of testosterone therapy did not improve survival either alone or with hepatocyte transplantation. In this study, hepatectomy exceeding 90% was lethal and did not respond to the supportive measures provided. Hepatocyte transplantation and testosterone pretreatment, both therapies which are thought to increase regeneration, were ineffective in improving survival in this resection model.