APPLICATION OF AN IMMUNOASSAY FOR CYCLIC ACROLEIN DEOXYGUANOSINE ADDUCTS TO ASSESS THEIR FORMATION IN DNA OF SALMONELLA-TYPHIMURIUM UNDER CONDITIONS OF MUTATION-INDUCTION BY ACROLEIN
APPLICATION OF AN IMMUNOASSAY FOR CYCLIC ACROLEIN DEOXYGUANOSINE ADDUCTS TO ASSESS THEIR FORMATION IN DNA OF SALMONELLA-TYPHIMURIUM UNDER CONDITIONS OF MUTATION-INDUCTION BY ACROLEIN
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DOI：
10.1093/carcin/10.1.87
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发表时间：
1989-01-01
期刊：
影响因子：
4.7
通讯作者：
CHUNG, FL
中科院分区：
文献类型：
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作者：
FOILES, PG;AKERKAR, SA;CHUNG, FL
丙烯醛已被证明在体外与 DNA 反应时会形成环状脱氧鸟苷加合物。在这项研究中，我们使用了最近开发的针对这些加合物的免疫测定法来研究它们在暴露于丙烯醛的鼠伤寒沙门氏菌 DNA 中的形成。丙烯醛-脱氧鸟苷加合物在沙门氏菌测试菌株TA100和TA104中以剂量依赖性方式形成，达到每摩尔脱氧鸟苷5μmol加合物的水平。使用液体预孵育测定，在菌株 TA100 和 TA104 中也发现了丙烯醛诱导的突变。丙烯醛-脱氧鸟苷加合物浓度与丙烯醛诱导的TA100突变（其在回复位点含有GC碱基对）之间的相关性表明丙烯醛-脱氧鸟苷加合物是一种促突变损伤。在回复位点含有 AT 碱基对的 TA104 中也发现了这种突变，这表明脱氧鸟苷以外的碱基加合物在丙烯醛的诱变活性中也可能很重要。
Acrolein has been shown to form cyclic deoxyguanosine adducts when it reacts with DNA in vitro. In this study, we have used a recently developed immunoassay for these adducts to study their formation in DNA from Salmonella typhimurium exposed to acrolein. Acrolein-deoxyguanosine adducts were formed in a dose-dependent fashion in Salmonella tester strains TA100 and TA104, reaching levels as high as 5 .mu.mol adduct per mol deoxyguanosine. Using the liquid pre-incubation assay, acrolein-induced mutations were also found in strains TA100 and TA104. The correlation between acrolein-deoxyguanosine adduct concentration and acrolein-induced mutations in TA100, which contains GC base pairs at the site of reversion, suggests that the acrolein-deoxyguanosine adduct is a promutagenic lesion. That mutations are also seen in TA104 which contains AT base pairs at the site of reversion suggest that adducts of bases other than deoxyguanosine may also be important in the mutagenic activity of acrolein.