Tissue distribution of the human CD97 EGF-TM7 receptor
Tissue distribution of the human CD97 EGF-TM7 receptor
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DOI：
10.1034/j.1399-0039.2001.057004325.x
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发表时间：
2001-04-01
期刊：
影响因子：
--
通讯作者：
Hamann, J
中科院分区：
文献类型：
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作者：
Jaspars, LH;Vos, W;Hamann, J
CD97是第二类七跨跨膜(7-TM)受体中EGF-TM7家族的创始成员。CD97有一个扩展的胞外区，含有几个N-末端表皮生长因子(EGF)样结构域，介导与CD55的结合。以往的研究表明，CD97表达于活化的淋巴细胞、单核细胞、巨噬细胞、粒细胞以及大量的造血细胞和非造血细胞系。在此，我们用免疫组织化学M法和免疫荧光(IF)法原位测定了人CD97的细胞分布。除小胶质细胞外，CD97在所有类型的巨噬细胞和树突状细胞上均有丰富的表达，在淋巴系中，大多数T细胞表达CD97，只有少数B细胞表达CD97。生发中心B细胞不表达该分子。除平滑肌细胞外，免疫系统外的其他细胞均未见染色。然而，对一组有限的上皮性肿瘤的分析显示，CD97在甲状腺和胃肠道癌症的恶性细胞上表达。
CD97 is a founding member of the EGF-TM7 family of class II seven-span transmembrane (7-TM) receptors. CD97 has an extended extracellular region with several N-terminal epidermal growth factor (EGF)-like domains, which mediate binding to CD55. Previous studies demonstrated the expression of CD97 on activated lymphocytes, monocytes, macrophages, granulocytes, and numerous haematopoietic and nonhaematopoietic cell lines. Here, we determined the cellular distribution of human CD97 in situ by immunohistochemistry M and immunofluorescence (IF). Abundant expression of CD97 was detected on all types of macrophages and dendritic cells, except for microglia, Within the lymphoid lineage, most T cells but only a few B cells express CD97. Germinal centre B cells do not express the molecule. Except for smooth muscle cells, no staining was found on other cells outside the immune system. However, analysis of a restricted set of epithelial tumors revealed CD97 expression on the malignant cells in thyroid and gastrointestinal tract cancer.