Pituitary-Derived Circular RNAs Expression and Regulatory Network Prediction During the Onset of Puberty in Landrace x Yorkshire Crossbred Pigs
Pituitary-Derived Circular RNAs Expression and Regulatory Network Prediction During the Onset of Puberty in Landrace x Yorkshire Crossbred Pigs
复制标题
长白猪和约克夏杂交猪青春期开始时垂体衍生的环状 RNA 表达和调节网络预测
DOI：
10.3389/fgene.2020.00135
复制
发表时间：
2020-02-28
影响因子：
3.7
通讯作者：
Li, Jiaqi
中科院分区：
文献类型：
--
作者：
Chen, Zitao;Pan, Xiangchun;Li, Jiaqi
垂体作为下丘脑-垂体-卵巢（HPO）轴的中枢，在青春期的启动中起着关键作用。最近的研究表明，环状RNA（circRNA）可以作为miRNA海绵来调节动物的发育。然而，垂体来源的circRNA在猪第一次发情中的功能仍不清楚。在这项研究中，我们使用长白猪×约克郡杂交猪的垂体在三个阶段（青春期前、青春期中和青春期后）进行了全基因组鉴定和表征circRNA，以描述猪垂体来源的circRNA。在后备母猪垂体中共检测到5148个circRNA，平均基因组距离为18682 bp，其中外显子circRNA约占91%，基因间circRNA约占6%，内含子circRNA约占3%。此外，首次鉴定了158种新的circRNA，并将其归类为推定的垂体特异性circRNA。它们在青春期开始时的表达水平显着超过其他circRNA，并且这些推定的垂体特异性circRNA的亲本基因富集在“ssc 04917：催乳素信号通路”、“ssc 04080：神经活性配体-受体相互作用”和“ssc 04728：多巴胺能突触”通路中，所有这些都与垂体功能一致。此外，通过构建以circRNA为靶点的miRNA-基因网络，发现并研究了17种差异调节的circRNA与miRNA以及沿着基因的潜在相互作用。综上所述，这些结果提供了新的见解circRNA介导的青春期母猪在垂体水平的时间。
Being the center of the hypothalamus-pituitary-ovary (HPO) axis, the pituitary plays a key role in the onset of puberty. Recent studies show that circular RNAs (circRNAs) can perform as miRNA sponges to regulate development in animals. However, the function of pituitary-derived circRNAs in first estrus remains unclear in pigs. In this study, we performed a genome-wide identification and characterization of circRNAs using pituitaries from Landrace × Yorkshire crossbred pigs at three stages: pre-, in-, and post-puberty, to describe such pituitary-derived circRNAs in pigs. A total of 5148 circRNAs were found in the gilts' pituitaries, averaging 18 682 bp in genomic distance, which consisted of approximately 91% exonic, 6% intergenic, and 3% intronic circRNAs. Furthermore, 158 novel circRNAs were identified for the first time and classified as putative pituitary-specific circRNAs. Their expression levels during the onset of puberty, significantly exceeded those of the other circRNAs, and the parental genes of these putative pituitary-specific circRNAs were enriched in “ssc04917: prolactin signaling pathway,” “ssc04080: neuroactive ligand-receptor interaction,” and “ssc04728: dopaminergic synapse” pathways, all of which were consistent with pituitary functioning. Additionally, 17 differentially regulated circRNAs were found and investigated for their potential interaction with miRNAs, along with genes, by constructing a circRNA-targeted miRNA-gene network. Taken together, these results provide new insight into the circRNA-mediated timing of puberty in gilts at the pituitary level.