GLT-1 expression and Glu uptake in rat cerebral cortex are increased by phencyclidine
GLT-1 expression and Glu uptake in rat cerebral cortex are increased by phencyclidine
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DOI：
10.1002/glia.20700
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发表时间：
2008-09-01
期刊：
影响因子：
6.2
通讯作者：
Conti, Fiorenzo
中科院分区：
文献类型：
--
作者：
Fattorini, Giorgia;Melone, Marcello;Conti, Fiorenzo
使用蛋白质印迹法，微透析，和功能测定，我们测试的假设，苯环利定（PCP）修改谷氨酸（Glu）转运体在大鼠额叶皮层的表达和功能。蛋白质印迹法研究显示，PCP（10 mg/kg/天; 7天）给药显著增加了星形胶质细胞Glu转运蛋白GLT-1/EAAT 2的表达。功能研究表明，PCP显着增加钠+依赖的谷氨酸吸收切片和神经元/星形胶质细胞共培养，和微透析研究证明，PCP治疗减少基础谷氨酸输出。在我们的实验条件下，五氯苯酚没有诱导毒性。这些研究表明，PCP增加了大脑皮层中GLT-1的表达，从而增加了Glu摄取并减少了细胞外[Glu]。(C)2008 Wiley-Liss，Inc.
Using western blottings, microdialysis, and functional assays we tested the hypothesis that phencyclidine (PCP) modifies the expression and function of glutamate (Glu) transporters in the rat frontal cortex. Western blotting studies revealed that administration of PCP (10 mg/kg/day; 7 days) increased significantly the expression of the astrocytic Glu transporter GLT-1/EAAT2. Functional studies showed that PCP increased significantly Na+-dependent Glu uptake in slices and in neuron/astrocyte co-cultures, and microdialysis studies evidenced that PCP treatment reduced basal Glu output. In our experimental conditions, PCP did not induce toxicity. These studies show that PCP increases the expression of GLT-1 in the cerebral cortex, thereby increasing Glu uptake and reducing extracellular [Glu]. (C) 2008 Wiley-Liss, Inc.