Proton NMR and NOE structural and dynamic studies of larger proteins and nucleic acids aided by isotope labels: T4 lysozyme.
Proton NMR and NOE structural and dynamic studies of larger proteins and nucleic acids aided by isotope labels: T4 lysozyme.
复制标题
借助同位素标记：T4 溶菌酶，对较大蛋白质和核酸进行质子 NMR 和 NOE 结构和动态研究。
DOI：
10.1080/07391102.1987.10506372
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发表时间：
1987
影响因子：
4.4
通讯作者：
Redfield,AG
中科院分区：
文献类型：
--
作者：
McIntosh,LP;Dahlquist,FW;Redfield,AG
本文综述了直接观测含有13C或15N标记的大分子的质子核磁共振的方法。附加到标签上的质子的共振和Overhauser效应可以从剩余的重叠共振中编辑或过滤。当选择性地结合标记时，这导致了光谱的简化。在2D和相关方法中，标记的化学位移提供了第二个维度，这对于光谱区分和鉴定是有用的。这些方法对较大的蛋白质是有用的，我们描述了我们在T4溶菌酶(质量为18.7kD)的研究方面的进展，我们已经在其中发现了大量的共振。
This article reviews methods based on direct observation of proton NMR in macromolecules containing13C or15N labels. The resonances and Overhauser effects of protons attached to the labels can be edited or filtered from the remaining overlapping resonances. This leads to simplification of the spectra when labels are incorporated selectively. In 2D and related methods the label's chemical shift provides a second dimension which is useful for spectral differentiation and identification. The methods are useful for larger proteins and we describe our progress on studies of T4 lysozyme, mass 18.7 kD, in which we have already identified a large number of resonances.