Interferon-β treatment alters peripheral blood monocytes chemokine production in MS patients
Interferon-β treatment alters peripheral blood monocytes chemokine production in MS patients
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DOI：
10.1016/s0165-5728(02)00064-4
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发表时间：
2002-05-01
影响因子：
3.3
通讯作者：
Khoury, SJ
中科院分区：
文献类型：
--
作者：
Comabella, M;Imitola, J;Khoury, SJ
趋化因子引导白细胞募集至炎症部位，也可能参与幼稚 T 辅助细胞产生细胞因子的调节。通过胞浆内染色研究了经干扰素-β (IFN-β) 治疗的多发性硬化症 (MS) 患者、未经治疗的 MS 患者和健康对照者血单核细胞产生的趋化因子。在未刺激的条件下，未治疗的 MS 患者和对照组之间白细胞介素 8 (IL-8)、干扰素诱导蛋白 10 (IP-10)、干扰素-γ (Mig) 诱导的单核细胞因子 (Mig)、单核细胞趋化蛋白 1 (MCP-1) 和单核细胞趋化蛋白 3 (MCP-3) 的产生没有差异。与对照组和未经治疗的多发性硬化症患者相比，服用 IFN-β 的多发性硬化症患者 T 细胞激活后单核细胞产生的趋化因子减少。与其他趋化因子不同，与对照组相比，未经治疗的 NIS 患者中单核细胞产生的巨噬细胞炎症蛋白 1α (MIP-1α) 显着减少，并且 IFN-β 治疗使 MIP-1α 表达增加至对照组的水平。体外向外周血单核细胞 (PBMC) 培养物中添加 IFN-β1b 往往会减少 IL-8、IP-10、Mig、MCP-1 和 MCP-3 的产生，但不会减少 MIP-1α 的产生。这些发现表明 IFN-β 治疗可能对单核细胞趋化因子的产生产生不同的影响。必须进行纵向研究来证实这些观察结果。 (C) 2002 Elsevier Science B.V. 保留所有权利。
Chemokines direct the recruitment of leukocytes to inflammatory sites and may also participate in the regulation of cytokine production by naive T helper cells. Chemokine production by blood monocytes was investigated by intracytoplasmic staining in interferon-beta (IFN-beta)-treated multiple sclerosis (MS) patients, untreated MS patients, and healthy controls. Under unstimulated conditions, no differences in the production of interleukin-8 (IL-8), IFN-inducible protein 10 (IP-10), monokine induced by interferon-gamma (Mig), monocyte chemoattractant protein-1 (MCP-1), and monocyte chemoattractant protein-3 (MCP-3) were seen between untreated MS patients and controls. Chemokine production by monocytes following T cell activation was decreased in MS patients taking IFN-beta compared to controls and untreated MS patients. Unlike other chemokines, macrophage inflammatory protein-1alpha (MIP-1alpha) production by monocytes was significantly decreased in untreated NIS patients compared to controls, and IFN-beta treatment increased MIP-1alpha expression to the level seen in controls. In vitro addition of IFN-beta1b to peripheral blood mononuclear cells (PBMC) cultures tended to decrease the production of IL-8, IP-10, Mig, MCP-1, and MCP-3, but not of MIP-1alpha. These findings suggest that IFN-beta treatment may have a differential affect on chemokine production by monocytes. Longitudinal studies must be done to confirm these observations. (C) 2002 Elsevier Science B.V. All rights reserved.